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| 低盐胁迫对褐菖鲉幼鱼抗氧化酶和离子调节酶活性的影响 |
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蒋飞1,2, 施永海1,2, 徐嘉波1,2, 邓平平1,2, 刘永士1,2, 袁新程1,2, 于爱清1,2
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1.上海市水产研究所，上海 200433;2.上海市水产技术推广站，上海 200433
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| 摘要: |
| 【目的】研究褐菖鲉（Sebastiscus marmoratus）幼鱼肝脏抗氧化酶和鳃离子调节酶活性在低盐胁迫下的变化规律，探讨其适应盐度骤变的能力。【方法】用6，8，10和20（对照）g/L盐度的水处理褐菖鲉幼鱼，依次记为S6、S8、S10和S20，在处理0，24，48，72和96 h时随机选取4尾鱼，取肝脏和鳃，分别测定抗氧化酶（SOD、CAT和GSH-Px）和离子调节酶（NKA和CMA）的活性。【结果】S6、S8和S10组SOD、CAT和GSH-Px活性在低盐胁迫下均呈现先上升后下降的趋势；在低盐胁迫24 h时，S6、S8和S10组的SOD和CAT活性均显著高于S20对照组（P<0.05）；在低盐胁迫48 h时，S6、S8和S10组的GSH-Px活性均显著高于S20对照组（P<0.05）；S6、S8和S10组的MDA含量在24和72 h时均显著高于S20对照组（P<0.05）；在低盐胁迫96 h时，各低盐胁迫组与对照组MDA含量无显著性差异（P>0.05）。低盐胁迫下S6、S8和S10组的鳃NKA和CMA活性均呈现先上升后下降并趋于稳定的趋势；在低盐胁迫24，48和72 h时，S6和S8组的 NKA和CMA活性均显著高于S10和S20组（P<0.05）。【结论】在盐度骤降时，褐菖鲉幼鱼可以通过提高抗氧化酶和离子调节酶活性来应对环境胁迫；随着胁迫时间的延长，抗氧化酶和离子调节酶活性逐渐下降并趋于平稳。 |
| 关键词: 褐菖鲉 低盐胁迫 抗氧化酶 离子调节酶 |
| DOI：10. 13207/j. jnwafu. 2026. 10. 004 |
| 分类号: |
| 基金项目:上海市水产遗传资源保护和开发利用项目（沪农委〔2025〕170号）；上海领军人才项目（沪人社专2021-67号）；上海长江口主要经济水生动物人工繁育工程技术研究中心项目（13DZ2251800） |
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| Effects of low salinity stress on the antioxidase enzymes and ion⁃regulating enzymes in juvenile Sebastiscus marmoratus |
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JIANG Fei1,2, SHI Yonghai1,2, XU Jiabo1,2, DENG Pingping1,2, LIU Yongshi1,2, YUAN Xincheng1,2, YU Aiqing1,2
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1.Shanghai Fisheries Research Institute，Shanghai 200433，China;2.Shanghai Fisheries Technical Extension Station，Shanghai 200433，China
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| Abstract: |
| 【Objective】The study investigated the changes in the activities of antioxidant enzymes in the liver and ion-regulating enzymes in the gills of juvenile Sebastiscus marmoratus under low salinity stress，to explore its ability to adapt to sudden changes in salinity.【Method】The experiment comprised four salinity gradi-ent treatments of 6，8，10，and 20 g/L（control），denoted as S6，S8，S10，and S20 respectively. At 0，24，48，72，and 96 h，4 individuals were randomly selected，and their livers and gills were collected to determine the activities of antioxidant enzymes（SOD，CAT and GSH-Px）and ion-regulating enzymes（NKA and CMA）respectively.【Result】Under low salinity stress，the activities of SOD，CAT，and GSH-Px in S6，S8，and S10 groups initially increased and then decreased. After low salinity stress treatment for 24 hours，the activities of SOD and CAT in S6，S8，and S10 groups were significantly higher than those in the control group S20（P<0.05）.After low salinity stress treatment for 48 hours，the activities of GSH-Px in S6，S8，and S10 groups were significantly higher than those in the control group S20（P<0.05）.After low salinity stress treatment for 24 hours and 48 hours，the MDA content in S6，S8，and S10 groups was significantly higher than that in the control group S20（P<0.05）.After low salinity stress treatment for 96 hours，there was no significant difference in MDA content between the low salinity stress groups and the control group（P>0.05）.Under low salinity stress，the activities of NKA and CMA in the gills of the fish in S6，S8，and S10 groups initially increased，then decreased，and finally stabilized. After low salinity stress treatment for 24 hours，48 hours，and 72 hours，the activities of NKA and CMA in S6 and S8 groups were significantly higher than those in S10 and S20 groups（P<0.05）.【Conclusion】When salinity drops abruptly，juvenile Sebastiscus marmoratus respond to environmental stress by enhancing the activities of antioxidant enzymes and ion-regulatory enzymes. However，as the duration of stress increases，the activities of these enzymes gradually decrease and finally stabilize. |
| Key words: Sebastiscus marmoratus low salinity stress antioxidant enzymes ion-regulating enzymes |
