| 摘要: |
| 【 目的】探究OsCOLD1基因在青萍抗寒中的功能及其对低温胁迫和ABA处理的响应机制，为青萍的 遗传改良及优异新种质创制提供参考。【 方法】以天津中医药大学中药学院中药资源实验室保存的青萍（Lemna turionifera，品系5511）为研究材料，采用愈伤组织诱导获得再生能力强、状态良好的青萍愈伤组织，利用农杆菌介导的遗 传转化技术在野生青萍中实现OsCOLD1基因过表达，系统分析低温胁迫和ABA处理对转基因青萍生长、抗寒性及 淀粉合成的影响。【 结果】筛选获得3个OsCOLD1基因过表达青萍株系，分别为OsCOLD1-4、OsCOLD1-7和OsCOLD1-8，其中OsCOLD1-4株系中OsCOLD1基因的相对表达量最高。低温胁迫下，OsCOLD1过表达株系的抗寒性 显著增强，淀粉含量显著提高，表明OsCOLD1可能通过调控代谢途径增强了青萍的低温耐受性和能量储备能力。 ABA处理下，野生型青萍和转基因青萍的生物量和淀粉含量均增加，但与野生型相比，OsCOLD1过表达青萍株系的 生物量和淀粉含量提升更为显著，推测OsCOLD1可能通过GPCR结构域与ABA结合，激活下游信号通路，进而调控 淀粉合成相关基因的表达。【 结论】OsCOLD1基因在青萍抗寒性和淀粉合成中发挥重要作用，为青萍遗传改良和优 异新种质创制提供了重要的基因资源和理论依据。 |
| 关键词: 青萍 OsCOLD1 基因 抗寒性 低温胁迫 |
| DOI：10. 13207/j. cnki.jnwafu. 2026. 05. 018 |
| 分类号: |
| 基金项目:国家自然科学基金项目（82074120）；秦创原中医药产业创新聚集区项目（L2024-QCY-ZYYJJQ-X49） |
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| Effects of OsCOLD1 gene overexpression on cold resistance and starch content of Lemna turionifera |
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YANG Pu1, JIA Rong1, LIU Wenying1, HE Shuping1, LIU Jingyan2, XU Xiaofan1, WANG Haiying1, XIANG Beibei1
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1.School of Chinese Materia Medica，Tianjin University of Traditional Chinese Medicine，Tianjin 301617，China;2.School of Agronomy & Resources and Environment，Tianjin Agricultural College，Tianjin 300384，China
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| Abstract: |
| Objective】This research aims to investigate the role of OsCOLD1 gene in cold tolerance of Lemna turionifera and its response mechanism to low temperature stress and ABA treatment，to provide reference for the genetic improvement of L. turionifera and the creation of excellent new germplasm.【Method】With L. turionifera（strain 5511） preserved in the Laboratory of Traditional Chinese Medicine Resources of the School of Chinese Materia Medica，Tianjin University of Traditional Chinese Medicine as the research material， the healing tissue induction was used to obtain L. turionifera healing tissues with strong regeneration ability and in good condition. The overexpression of OsCOLD1 gene was realized in L. turionifera by using the Agrobacte?rium-mediated genetic transformation technology. The effects of low temperature and ABA treatments on the growth，cold resistance and starch synthesis of transgenic L. turionifera were systematically analyzed.【Result】Three overexpression lines of L. turionifera，namely OsCOLD1-4，OsCOLD1-7 and OsCOLD1-8，were obtained，among which OsCOLD1-4 showed the highest expression level of OsCOLD1 gene. Under low-tempera-ture stress，OsCOLD1-4 overexpression lines showed significant increase in cold tolerance and starch content，suggesting that OsCOLD1 may enhance the low-temperature tolerance and energy reserve capacity of L. turionifera by regulating metabolic pathways. The biomass and starch content of wild-type and transgenic L. turionifera were increased under ABA treatment. However，compared to the wild type，the OsCOLD1 overexpressing lines showed significantly greater increases in biomass and starch content. It was hypothesized that OsCOLD1 might bind to ABA through the GPCR domain and activate the downstream signa-ling pathway，thus regulating the expression of starch synthesis-related genes.【Conclusion】OsCOLD1 gene plays an important role in cold resistance and starch synthesis of L. turionifera，which provides important genetic resources and theoretical basis for genetic improvement and creation of new germplasm of L. turionifera. |
| Key words: Lemna turionifera OsCOLD1 gene cold resistance low-temperature stress |
