| 摘要: |
| 【目的】明确引起呼和浩特市庭院及包头市农牧科学研究所朱顶红育种基地朱顶红（Hippeastrum rutilum）叶片褪绿及花叶病的病毒种类，扩增其全基因组序列并分析其遗传多样性。【方法】以2023年5月从内蒙古自治区呼和浩特市庭院和包头市农牧科学研究所朱顶红育种基地采集的320份疑似感染病毒的朱顶红叶片为试验材料，通过高通量测序（HTS）技术明确造成该病害的病毒性病原为尼润潜隐病毒（nerine latent virus，NeLV），通过RT-PCR技术和cDNA末端快速扩增（RACE）技术获得NeLV的全基因组序列，并将其命名为23-HiRu1。利用SDTv1.3软件、MEGA 11.0软件中的最大似然法以及RDP4和DnaSP软件对其进行序列一致性、系统发育、重组分析以及遗传多样性分析。【结果】23-HiRu1基因组全长为8 270 nt（GenBank登录号：PQ261105），编码6个开放阅读框（ORFs），与已报道的NeLV基因组特征一致。该分离物与已报道的NeLV分离物全基因组核苷酸序列一致性为75.4%~97.9%，CP 基因核苷酸和氨基酸一致性分别为78.3%~98.2%和90.2%~99.7%，符合ICTV对香石竹潜隐病毒属（Carlavirus）种的划分标准。系统发育分析结果显示，NeLV各分离物可划分为2个亚群，其中本研究所获的分离物23-HiRu1在Group Ⅰ中，且23-HiRu1与南非共和国朱顶红分离物Amaryllis-1 isolate 19-3029亲缘关系最近。遗传多样性分析结果表明，NeLV具有较高的遗传多样性，整体受到了负选择压力，地理特异性明显，未发现重组事件。【结论】引起内蒙古朱顶红叶片褪绿和花叶病的病原为尼润潜隐病毒（NeLV），其与南非共和国朱顶红分离物Amaryllis-1 isolate 19-3029的亲缘关系最近；NeLV具有较高遗传多样性，受较强负选择压力，地理特异性明显，未发现重组事件。该结果为NeLV的分子特征和遗传进化研究提供了重要依据。 |
| 关键词: 尼润潜隐病毒 朱顶红 全基因组序列 系统发育分析 遗传多样性分析 |
| DOI：10.13207/j.jnwafu.2026.02.013 |
| 分类号: |
| 基金项目:内蒙古自治区自然科学基金项目（2022QN03018，2023LHMS03020）；内蒙古自治区高等学校青年科技英才项目（NJYT23079）；内蒙古自治区直属高校基本科研业务费项目（BR230128） |
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| Complete genome sequence amplification and genetic diversity analysis of nerine latent virus Inner Mongolia isolate |
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GAO Jinyu1, GUO Mengze1, ZHAO Xue1, SUN Pingping1, ZHANG Lei1, BAI Yinfeng2, LI Zhengnan1
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1.College of Horticulture and Plant Protection，Inner Mongolia Agricultural University，Hohhot，Inner Mongolia 010018，China;2.Baotou Institute of Agriculture and Animal Husbandry Science and Technology，Baotou，Inner Mongolia 014010，China
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| Abstract: |
| 【Objective】This research aims to identify the virus species that cause leaf chlorosis and mosaic virus disease in Hippeastrum rutilum in the courtyard of Hohhot and in the Zhudinghong breeding base of Baotou Institute of Agricultural and Animal Husbandry Science and Technology,amplify their whole genome sequences,and analyze their genetic diversity.【Method】With 320 suspected virus-infected leaves collected from the courtyard of Hohhot City and Zhudinghong breeding base of Baotou Institute of Agricultural and Animal Husbandry Science and Technology in Inner Mongolia Autonomous Region in May 2023 as samples,the viral pathogen causing the disease was identified as nerine latent virus (NeLV) through high throughput sequencing (HTS) technology.The complete genome sequence of NeLV was obtained through RT-PCR and rapid amplification of cDNA ends (RACE) technology,and was named as 23-HiRu1.Sequence consistency,phylogenetic,recombination analysis,and genetic diversity analysis were performed by using SDTv1.3 software,maximum likelihood method in MEGA 11.0 software,and RDP4 and DnaSP software.【Result】The total length of the 23-HiRu1 genome is 8 270 nt (GenBank accession number:PQ261105),encoding 6 ORFs that are consistent with the reported NeLV genome features.The nucleotide sequence consistency of this isolate with the reported NeLV isolate is 75.4%-97.9%,and the nucleotide and amino acid consistency of the CP gene is 78.3%-98.2% and 90.2%-99.7%,respectively,which meets the ICTV classification criteria for the genus Carlavirus in Caryophyllum.The phylogenetic analysis results showed that the NeLV isolates can be divided into two subgroups.Among them,the isolate 23-HiRu1 obtained in this study belongs to Group Ⅰ,and 23-HiRu1 has the closest genetic relationship with the South African red isolate Amaryllis-1 isolate 19-3029.The results of genetic diversity analysis indicate that NeLV has high genetic diversity and obvious geographic specificity,which is subjected to negative selection pressure as a whole,with no recombination events found.【Conclusion】This study identified the pathogen of red leaf chlorosis and mosaic virus disease in Inner Mongolia as NeLV.NeLV is most closely related to Amaryllis-1 isolate 19-3029 from the Republic of South Africa.NeLV has high genetic diversity and significant geographic specificity,which is subject to strong negative selection pressure,with no recombination events found.The study provides important evidence for the molecular characteristics and genetic evolution of NeLV. |
| Key words: nerine latent virus（NeLV） Hippeastrum rutilum full genomic sequence phylogenetic analysis genetic diversity analysis |
