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褪黑素对枇杷果实贮藏品质的影响
周甲云1, 谭碧其1, 王羊2, 原贞超1, 汤开晶3, 郑世玉3, 李红章4, 邓群仙1
1.四川农业大学 园艺学院,四川 成都 611130;2.成都农业职业科技学院 农学园艺学院,四川 成都611130;3.石棉县农业农村局,四川 雅安625000;4.仁寿县农业农村局经济作物站,四川 眉山 620599
摘要:
【目的】探究外源褪黑素对枇杷果实低温贮藏期间及货架期品质的影响,以期筛选出适宜的褪黑素浓度,为枇杷果实贮藏保鲜提供参考。【方法】以‘大五星’枇杷成熟果实为试验材料,分别用0(蒸馏水,对照),50,100,150和200 μmol/L褪黑素浸果1 h,4 ℃贮藏30 d后再转至20 ℃贮藏3 d,测定果实腐烂率、失重率、硬度、内聚性及可溶性固形物(TSS)、可溶性糖、可滴定酸(TA)、类胡萝卜素、总酚、丙二醛(MDA)、木质素含量,冷害指数、相对电导率,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)、苯丙氨酸解氨酶(PAL)、肉桂酸脱氢酶(CAD)、过氧化物酶(POD)活性,分析果实生理与相关品质指标的变化。【结果】褪黑素处理可降低枇杷贮藏期间果实的失重率、腐烂率及硬度,维持其内聚性;100 μmol/L褪黑素处理的果实在低温贮藏至30 d,果实可溶性固形物、可溶性糖、可滴定酸、总酚和类胡萝卜素含量较对照分别提高了6.9%,19.1%,41.9%,69.9%和38.5%,转至货架期(贮藏33 d)时较对照分别提高了10.4%,39.0%,95.3%,73.8%和41.3%。褪黑素处理降低了果实贮藏期间冷害指数、相对电导率和MDA含量,维持了SOD、CAT和APX活性;与对照相比,低温贮藏至30 d时,100 μmol/L褪黑素处理后果实的相对电导率和MDA含量降幅最大,分别为15.1%和17.3%;SOD和CAT活性在贮藏期间先升高后降低,贮藏30和33 d时,褪黑素处理果实SOD、CAT和APX活性较对照显著提高,其中100 μmol/L处理的增幅最大。褪黑素通过抑制枇杷果实贮藏中后期的PAL、CAD和POD活性,降低了果实木质素含量,其中100 μmol/L褪黑素处理果实在低温贮藏末期和货架期木质素含量显著低于对照。【结论】褪黑素浸果处理可有效维持枇杷果实在贮藏期间的外观品质、生理特性和营养品质,以100 μmol/L处理效果最佳。
关键词:  褪黑素  枇杷果实  贮藏品质  低温贮藏期  货架期
DOI:10.13207/j.jnwafu.2026.01.006
分类号:
基金项目:四川省科技支撑计划“十四五”育种攻关项目(2021YFYZ0023-07);国家现代化农业产业技术体系四川水果创新团队项目(SCCXTD-2024-04)
Effect of melatonin on the quality of loquat fruit during storage
ZHOU Jiayun1, TAN Biqi1, WANG Yang2, YUAN Zhenchao1, TANG Kaijing3, ZHENG Shiyu3, LI Hongzhang4, DENG Qunxian1
1.College of Horticulture,Sichuan Agricultural University,Chengdu,Sichuan 611130,China;2.College of Agronomy and Horticulture,Chengdu Agricultural College,Chengdu,Sichuan 611130,China;3.Shimian County Agriculture and Rural Bureau,Yaan,Sichuan 625000,China;4.Renshou County Agriculture and Rural Bureau Cash Crop Station,Meishan,Sichuan 620599,China
Abstract:
【Objective】This research aimed to explore the effects of exogenous melatonin on the quality of ‘Dawuxing’ loquat fruits during low temperature storage and shelf life,in order to screen out the appropriate melatonin concentration,to provide reference for the storage of loquat fruits.【Method】Loquat fruits were soaked in 0 (distilled water,CK), 50,100,150,and 200 μmol/L melatonin for 1 h,and stored at 4 ℃ for 30 days and then at 20 ℃ for 3 days.The fruit decay rate,weight loss rate,hardness,cohesion,soluble solid (TSS) content,soluble sugar content,titrable acid (TA) content,carotenoid content,total phenol content,malondialdehyde (MDA) content, lignin content,cold damage index,relative conductivity,and activities of superoxide dismutase (SOD),catalase (CAT),ascorbate peroxidase (APX),phenylalanine ammonlyase (PAL),cinnamate dehydrogenase (CAD),and peroxidase (POD) were determined to analyze the physiological changes and changes of related quality indexes.【Result】Melatonin treatment can reduce the weight loss rate,decay rate and hardness of loquat fruits during storage,and maintain their cohesion.The contents of soluble solid matter,soluble sugar,titrable acid,total phenol and carotenoid in fruits treated with 100 μmol/L melatonin were increased by 6.9%,19.1%,41.9%,69.9% and 38.5%,respectively,compared with the control when stored at low temperature for 30 days,and were increased by 10.4%,39.0%,95.3%,73.8% and 41.3%,respectively,compared with the control when the fruits were transferred to shelf (stored for 33 days).Melatonin treatment decreased the chilling damage index,relative conductivity and malondialdehyde (MDA) content of fruit during storage, and maintained the activities of superoxide dismutase (SOD),catalase (CAT) and ascorbate peroxidase (APX).Compared with the control, the relative conductivity and MDA content of fruits treated with 100 μmol/L melatonin showed the largest decrease after low temperature storage for 30 days,with reductions of 15.1% and 17.3%,respectively.SOD and CAT activities first increased and then decreased during storage.SOD,CAT and APX activities of fruit treated with melatonin were significantly higher than those of control at 30 d and 33 d of storage,with the largest increase observed in treatment of 100 μmol/L melatonin.Melatonin inhibited the activities of phenylalanine ammoniase (PAL),cinnamate dehydrogenase (CAD) and peroxidase (POD) in loquat fruits in the middle and later stages of storage,and the lignin content in fruits treated with 100 μmol/L melatonin was significantly lower than that in the control at the end of low temperature storage and shelf life.【Conclusion】Melatonin soaking can effectively maintain the appearance quality,physiological characteristics and nutritional quality of loquat fruit during storage,and 100 μmol/L melatonin has the best effect.
Key words:  melatonin  loquat fruit  storage quality  low temperature storage period  shelf life