| 摘要: |
| 【目的】研究低温胁迫下番茄幼苗MYB转录因子家族基因对光合特性的调控作用，为进一步阐明其与植物抗寒性的关系提供参考。【方法】以番茄品种‘Micro Tom’野生型植株(WT)及SlMYB1R1过表达株系(SlMYB1R1-8、SlMYB1R1-14)为试验材料，测定4 ℃低温胁迫5 d后番茄幼苗叶绿素含量、光合气体交换参数、叶绿素荧光参数以及逆境响应基因(SlCBF1、SlCBF2、SlCBF3、SlDRCI7)的表达水平。【结果】4 ℃低温胁迫5 d后，所有番茄植株均出现萎焉现象，但SlMYB1R1过表达植株萎焉程度较轻，常温下恢复程度较好。低温胁迫导致所有番茄植株的净光合速率、气孔导度、蒸腾速率以及叶绿素荧光参数大幅下降，但SlMYB1R1过表达植株的上述指标均显著高于WT植株。在低温胁迫前后,番茄幼苗SlMYB1R1基因以及低温逆境响应基因表达水平均显著高于野生型植株。【结论】过表达SlMYB1R1能够缓解低温胁迫对番茄幼苗光合作用的抑制，同时显著上调其低温逆境响应基因的表达水平，从而提高番茄植株的抗寒能力。 |
| 关键词: 番茄 低温胁迫 SlMYB1R1 光合参数 |
| DOI：10.13207/j.cnki.jnwafu.2025.11.014 |
| 分类号: |
| 基金项目:四川省科技厅面上项目(2024NSFSC0396) |
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| Effects of overexpressing SlMYB1R1 on photosynthetic properties of tomato seedlings under low temperature stress |
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LI Jiajia, ZHAO Ruozhu, YANG Wenwen, XIE Can, NIE Shuming
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Key Laboratory of Southwest China Wildlife Resources Conservation,College of Life Science,China West Normal University,Nanchong,Sichuan 637000,China
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| Abstract: |
| 【Objective】The objective of this study is to examine the regulatory effects of the MYB transcription factor family genes on photosynthetic characteristics of tomato seedlings under low temperature stress.This study provides guidance for understanding the roles of the MYB transcription factor genes in regulating cold resistance.【Method】Wild-type (WT) plants and SlMYB1R1 overexpressing lines (SlMYB1R1-8 and SlMYB1R1-14) of tomato cultivar ‘Micro Tom’ were used as experimental materials.We determined the chlorophyll content,photosynthetic gas exchange parameters,chlorophyll fluorescence parameters,and expression of stress response genes (SlCBF1,SlCBF2,SlCBF3, and SlDRCI7) of tomato seedlings at 4 ℃ for 5 days.【Result】After 5 days of low temperature stress,all tomato plants became wilting,but the wilting degree of SlMYB1R1 overexpressing plants was lighter,and the recovery degree was better at room temperature.Low temperature stress decreased net photosynthetic rate,stomatal conductance,transpiration rate,and chlorophyll fluorescence parameters of all tomato plants,but SlMYB1R1 overexpressing plants were higher than WT plants.The expression levels of SlMYB1R1 and low temperature stress response genes in SlMYB1R1 overexpressing plants were higher than WT plants before and after low temperature stress.【Conclusion】Overexpression of SlMYB1R1 can improve cold resistance of tomato plants by alleviating photosynthesis reduction and up-regulating the expression of cold stress response genes under low temperature stress. |
| Key words: Solanum lycopersicum L. low temperature stress SlMYB1R1 photosynthetic parameters |
