| 摘要: |
| 【目的】从米槁根际土壤筛选出具有较好促生作用的根际促生真菌（plant growth promoting fungi,PGPF）以及最佳的菌株复配方案，为缓解米槁资源短缺提供技术支撑。【方法】米槁根际土壤中的真菌使用马铃薯葡萄糖琼脂培养基（PDA）进行分离纯化，利用功能性培养基筛选出具有溶磷、解钾、产吲哚-3-乙酸（IAA）、产嗜铁素促生能力的真菌,采用平板培养法对同一促生功能及不同促生功能菌株进行对峙培养，观察菌株在对峙培养期间的形态和群落变化，并测定对峙培养菌株的脲酶、β-葡萄糖苷酶、酸性磷酸酶、FDA水解酶活性。【结果】从米槁根际土壤中共分离得到真菌109种，其中具有较强促生能力的真菌14种，Nectria pseudocinnabarina、爪哇虫草菌（Cordyceps javanica）、Purpureocillium lilacinum、Gonytrichum macrocladum具有溶磷能力，Sordariomycetes sp.、Hypocrea lixii、嗜粪青霉（Penicillium coprophilum）具有解钾能力，Cordana terrestris、Kiflimonium curvulum、Hypocrea lixii、腐皮镰刀菌（Fusarium solani）、绿僵菌（Metarhizium carneum）、波氏假性阿利什霉（Pseudallescheria boydii）具有产嗜铁素能力，且所有菌株具有产IAA能力。Sordariomycetes sp.、Purpureocillium lilacinum、Kiflimonium curvulum三者在对峙培养时，脲酶、β-葡萄糖苷酶、酸性磷酸酶和FDA水解酶活性均显著升高，且无明显拮抗现象。【结论】Sordariomycetes sp.、Purpureocillium lilacinum和Kiflimonium curvulum 3种菌株复配效果较为理想，可成为未来米槁根际促生菌肥开发试验的候选菌种。 |
| 关键词: 米槁 根际促生菌 拮抗作用 对峙培养 酶活性 |
| DOI：10.13207/j.cnki.jnwafu.2025.04.014 |
| 分类号: |
| 基金项目:贵州省基础研究项目（黔科合基础-ZK［2022］一般314）；贵州省科技支撑项目（黔科合支撑［2023］一般049）；贵州中医药大学博士启动基金项目（贵中医启动［2023］31号）；贵州省教育厅高等学校自然科学研究项目（黔教技［2024］121号） |
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| Antagonistic effect of fungi in the rhizosphere soil of Cinnamomum migao |
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CHEN Jingzhong1,2, PENG Liang3, LIAO Xiaofeng4, LIU Jiming5, TONG Bingli6
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1.Traditional Chinese Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550025,China;2.Guizhou Key Laboratory for Raw Material of Traditional Chinese Medicine,Guiyang,Guizhou 550025,China;3.Agricultural Service Center in Yangjia Town,Peng’an County,Nanchong,Sichuan 637800,China;4.Institute of Mountain Resources,Guizhou Academy of Sciences,Guiyang,Guizhou 550025,China;5.Guizhou Academy of Forestry,Guizhou University,Guiyang,Guizhou 550025,China;6.School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550025,China
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| Abstract: |
| 【Objective】The study aimed to obtain plant growth promoting fungi (PGPF) with superior growth-promoting effects and the optimal strain combination scheme from the rhizosphere fungi of Cinnamomum migao seedlings,thereby providing technical support for alleviating the shortage of C. migao resources.【Method】The fungi in the rhizosphere soil of C. migao were isolated and purified using PDA medium,and a functional medium was used to screen fungi with phosphorus solubilization,potassium solubilization,IAA production,and siderophore promoting abilities.The plate culture method was adopted to cultivate strains either with the same or different growth promoting functions in a paired culture.During the confrontational culture period,the morphological and community changes of the strains were observed,while the urease activity,as well as the β glucosidase,acid phosphatase,and fluorescein diacetate hydrolase activities of the confrontational culture strains were measured. 【Result】A total of 109 fungi were isolated from the rhizosphere soil of C. migao,with 14 fungi exhibiting strong growth promoting ability.Among these,Nectria pseudocinnabarina,Cordyceps javanica,Purpureocillium lilacinum,and Gonytrichum macrocladum possessed phosphorus solubilization ability.Additionally,Sordariomycetes sp.,Hypocrea lixii,and Penicillium coprophilum exhibited potassium solubilization ability.Cordana terrestris,Kiflimonium curvulum,Hypocrea lixi,Fusarium solani,Metarhizium carneum,and Pseudallescheria boydii exhibited iron producing ability,and all strains also possessed the capability to produce IAA.During the confrontational cultivation of Sortariomycetes sp.,Purpureocillium lilacinum,and Kiflimonium curvulum,the activities of urease,β-glucosidase,acid phosphatase,and fluorescein diacetate hydrolase all significantly increased without significant antagonism.【Conclusion】The combined formulation of Sordariomycetes sp.,Purpureocillium lilacinum,and Kiflimonium curvulum exhibits a better promoting effect.In the future,it may become a candidate solution for the development and experimentation of plant growth promoting fungi for C. migao. |
| Key words: Cinnamomum migao growth-promoting bacteria antagonistic effect confrontation culture enzyme activity |
