| 摘要: |
| 【目的】研究继代培养周期对牡丹试管苗不定根发生的影响及其生理机制，为牡丹试管苗工厂化生产提供参考。【方法】以‘正午’牡丹试管苗为材料，在增殖培养过程中定期(20，30，40，50，60 d) 选取单芽进行生根培养，测定试管苗生根指标(生根率、平均根数、平均根长)和不同培养时期内源激素吲哚乙酸(IAA)、脱落酸(ABA)、细胞分裂素(ZR)和赤霉素(GA3)含量，过氧化物酶(POD)、多酚氧化酶(PPO)、吲哚乙酸氧化酶(IAAO)活性，以及可溶性糖、可溶性蛋白和总酚含量，分析生根状况与生理指标的相关性。【结果】‘正午’牡丹继代培养40 d的试管苗生根效果最佳，此时的生根率(30.86%)显著高于其他处理，平均根数1.17条，平均根长0.83 cm，愈伤组织小，生根质量较高。继代培养过程中，内源激素IAA、ABA、ZR含量的动态变化一致，而GA3含量与之相反；IAA、ABA含量与平均根数呈显著正相关，而GA3含量与平均根数呈显著负相关；IAA/ZR、IAA/GA3与平均根数呈显著和极显著正相关。POD、PPO、IAAO活性波动较大，其中PPO活性与生根率呈显著负相关，IAAO活性与平均根数呈显著正相关。可溶性糖含量与生根率呈极显著正相关。【结论】‘正午’牡丹试管苗的最佳继代培养周期为40 d，该时期试管苗的生理状态更适宜生根，IAA、可溶性糖含量对试管苗生根的正向影响较大。 |
| 关键词: ‘正午’牡丹 继代培养周期 不定根 生根率 |
| DOI： |
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| 基金项目:国家自然科学基金项目 （32001359）; 南京林业大学大学生创新训练计划项目（2021NFU-SPITP0015） |
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| Effect of subculture cycle on in vitro adventitious rooting of Paeonia×lemoinei ‘High Noon’ and related physiological indexes |
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WEN Shusheng,CUI Huiying,SUN Xueying,et al
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| Abstract: |
| 【Objective】The influence of subculture cycle on in vitro adventitious rooting of peony and its physiological mechanism were studied to provide reference for factory production of peony seedlings.【Method】Single buds of Paeonia×lemoinei ‘High Noon’ in vitro were selected for root transfer culture periodically (20,30,40,50 and 60 days) during the proliferation and culture process.Rooting indicators of rooting rate,average number of roots and average root length,contents of endogenous hormones including indoleacetic acid (IAA),abscisic acid (ABA),zeatin riboside (ZR) and gibberellins (GA3),activities of enzymes such as peroxidase (POD),polyphenol oxidase (PPO) and indoleacetic acid oxidase (IAAO),and contents of soluble sugar,soluble protein and total phenol were measured and their correlations were analyzed.【Result】The rooting rate (30.86%) of Paeonia×lemoinei ‘High Noon’ in vitro seedlings subcultured for 40 days was significantly higher than other treatments.The treatment also had average number of roots of 1.17,average root length of 0.83 cm,smaller callus and higher rooting quality.During subculture,changes of IAA,ABA and ZR contents were identical,whereas GA3 exhibited an inverse trend.Contents of IAA and ABA were positively correlated with average number of roots.The content of GA3 was negatively correlated with average number of roots.IAA/ZR and IAA/GA3 had significantly positive correlation with average number of roots.Activities of POD,PPO and IAAO fluctuated greatly,among which PPO activity was negatively correlated with rooting rate,while IAAO activity was positively correlated with average number of roots.Soluble sugar content was positively correlated with rooting rate,while soluble protein content was not.There was no significant correlation between total phenol content and rooting indexes.【Conclusion】The length of 40 days was the best subculture period for Paeonia×lemoinei ‘High Noon’ in vitro with physiological state more suitable for rooting,and contents of IAA and soluble sugar had greater positive effects on seedling rooting. |
| Key words: Paeonia×lemoinei ‘High Noon’ subculture cycle root rooting rate |
