| 摘要: |
| 【目的】筛选与马铃薯主茎数和单株结薯数显著关联的单核苷酸多态性(SNP)位点并挖掘候选基因，为高产马铃薯分子育种提供基因资源。【方法】以251份马铃薯核心种质为材料，于2018-2019年在马铃薯主产区4个试验点共8个环境下对其主茎数和单株结薯数进行表型鉴定；提取251份马铃薯种质的DNA，并采用高通量IlluminaHiSeqTM对DNA进行测序及处理，获得高质量SNP标记，在此基础上，结合表型性状进行全基因组关联（GWAS）分析，对显著关联的SNP位点所在区域的候选基因通过NR、Swiss-port、KEGG、GO等4个数据库进行功能注释。【结果】2018-2019年的检测结果表明，在环境和基因型互作下，251份马铃薯种质间主茎数与单株结薯数有广泛的表型变异。共获得1 209 969个高质量SNP位点，其中与主茎数显著关联的SNP位点有7个，共挖掘到19个候选基因，其中在3个以上环境重叠的候选基因有9个，其可能编码半乳糖醛酸转移酶、转录因子MYB、赤霉素3-β-双加氧酶及细胞色素P450；与单株结薯数显著关联的SNP位点有13个，共挖掘到33个候选基因，去掉2年间重叠的4个候选基因，实际共关联到29个候选基因，其中在3个以上环境下重叠的候选基因有11个，其可能编码果糖-1,6-二磷酸醛缩酶、生长素响应因子IAA13、转录因子WRKY、bHLH113及MADS-box。【结论】在2年8个环境下共鉴定出20个与马铃薯主茎数和单株结薯数显著相关的SNP位点，挖掘到48个候选基因。 |
| 关键词: 马铃薯育种 主茎数 单株结薯数 全基因组关联分析 候选基因挖掘 |
| DOI： |
| 分类号: |
| 基金项目:国家马铃薯产业技术体系项目（CARS-09-ES03）；内蒙古农牧业科技创新基金项目（2018CXJJN01） |
|
| Candidate genes related to numbers of main stems and tubers per plant of potato mined using GWAS |
|
HAN Zhigang,XIE Rui,GUO Binyu,et al
|
| Abstract: |
| 【Objective】SNPs significantly related to numbers of main stems and tubers per plant of potato were screened and candidate genes were mined to provide gene resources for molecular breeding of high-yield potatoes.【Method】The phenotyping of a core collection of 251 potato germplasms for numbers of main stems and tubers per plant was carried out in 8 environments at 4 test sites in main potato production areas during 2018-2019.The genomic DNA was extracted,and high throughput sequencing was performed on IlluminaHiSeqTM platform for identification of high-quality SNPs markers.On this basis,genome-wide association (GWAS) analysis was carried out in combination with phenotypic data,and the candidate genes in the regions surrounding associated SNP sites were annotated through four databases including NR,Swiss-port,KEGG and GO.【Result】With interactions of environment and genotype,251 potato germplasms showed extensive phenotypic variations in numbers of main stems and tubers per plant in 2018 and 2019.A total of 1 209 969 high quality SNP sites were obtained.Seven SNP sites were found to be significantly associated with the number of main stems,and a total of 19 candidate genes were mined.There were 9 candidate genes that overlapped in at least 3 different environments,which may encode galactosyltransferase,transcription factor MYB,and gibberellin 3-β-dioxygenase and cytochrome P450.There were 13 SNP sites significantly associated with the number of tubers per plant,and a total of 33 candidate genes were mined.Excluding the 4 candidate genes that overlapped in 2 years,29 candidate genes were associated.Among them,11 candidate genes overlapped in more than 3 environments,which may encode fructose-1,6-diphosphate aldolase,auxin response factor IAA13,transcription factors WRKY,bHLH113 and MADS-box.【Conclusion】 A total of 20 SNP loci significantly related to numbers of main stems and tubers per plant were identified,and 48 candidate genes were mined in 8 environments in 2 years. |
| Key words: potato breeding number of main stem tubers per plant genome-wide association analysis candidate gene mining |
