| 摘要: |
| 【目的】探究盐胁迫对土沉香中沉香四醇及2-(2-苯乙基)色酮的诱导作用。【方法】在传统冷钻和火钻的基础上增加盐（质量分数26% NaCl）胁迫，设冷钻、冷钻+盐、火钻、火钻+盐4个处理，结香10个月后，使用体积分数95%乙醇对各处理沉香样品进行超声提取得到醇提物，采用气相色谱-质谱(GC-MS)联用法对沉香的化学成分进行定性分析，使用超高效液相色谱串联三重四级杆质谱仪（UPLC-MS-MS）,测定其中沉香四醇及2-(2-苯乙基)色酮的绝对含量。【结果】① 冷钻和火钻结合盐处理后，沉香醇提物含量均不同程度提升，其中冷钻+盐处理醇提物含量较冷钻处理提高21.43%，火钻+盐处理醇提物含量较火钻处理提高64.86%。② 与冷钻和火钻处理相比，加盐处理后沉香中化合物种类总数未增加，冷钻和冷钻+盐处理化合物种类为35种，火钻和火钻+盐处理化合物种类为46种；冷钻+盐处理倍半萜类和色酮类物质种类与冷钻处理相比均未增加，火钻+盐处理倍半萜类化合物较火钻处理增加1种, 色酮类化合物较火钻处理增加4种;加盐处理后沉香中倍半萜类物质相对含量减少，色酮类物质相对含量增加，冷钻处理、冷钻+盐处理、火钻处理、火钻+盐处理沉香中倍半萜类物质相对含量分别为88.67%，75.34%，56.72%和54.31%，色酮类物质相对含量分别为8.74%，20.96%，27.05%和43.24%。③ 冷钻+盐处理沉香中沉香四醇、2-(2-苯乙基)色酮含量分别为1.08和0.14 mg/g，是冷钻处理（0.37和0.01 mg/g）的2.92和13.18倍；火钻+盐处理沉香中沉香四醇、2-(2-苯乙基)色酮含量分别为2.93和0.68 mg/g，是火钻处理（1.22和0.07 mg/g）的2.42和9.90倍。【结论】盐胁迫显著提升了沉香醇提物含量，增加了火钻处理的倍半萜类和色酮类物质种类，且对沉香四醇和2-(2-苯乙基)色酮的产生有诱导作用。 |
| 关键词: 沉香 盐胁迫 结香方法 沉香四醇 2-(2-苯乙基)色酮 |
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| 基金项目:广东省林业科技创新项目(2017KJCX001,2020KJCX007)；中央级公益性科研院所基本科研业务费专项资金项目(CA-FYBB2020SY019) |
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| Effects of salt stress on contents of agarotetrol and 2-(2-phenylethyl) chromone |
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LI Zhihong,ZHANG Ningnan,XU Daping,et al
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| Abstract: |
| 【Objective】This study investigated the inducing effect of salt stress on the production of agarotetrol and 2-(2-phenylethyl) chromone. 【Method】Based on traditional cold drilling and fire drilling,the salt stress using aqueous solution of NaCl with a mass fraction of 26% was added to set four treatments of cold drill,cold drill + salt,fire drill and fire drill + salt.After ten months,the extracts of 95% ethanol were obtained by ultrasonic ethanol extraction method. The chemical components of agarwood were qualitatively analyzed by gas chromatography-mass spectrometry (GC-MS) after agarwood formation, and ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS-MS) was used to determine the contents of agarotetrol and 2-(2-phenylethyl) chromone.【Result】① The alcohol extract content of cold drill + salt was increased by 21.43% compared with cold drill,and that of fire drill + salt was increased by 64.86% compared with fire drill.② Compared with cold drill and fire drill treatments,the total number of compounds in agarwood did not increase after adding salt.Cold drilling and cold drilling + salt treatments obtained 35 compounds, while fire drill and fire drill + salt treatments obtained 46 compounds.Compared with cold drill treatment, the types of sesquiterpenes and chromones did not increase after salting. The types of sesquiterpenoids in fire drill + salt treatment increased by 1 species compared with fire drill treatment and the types of chromone compounds in fire drill + salt treatment increased by 4 compared with fire drill treatment.After salting,the relative content of sesquiterpenes in agarwood decreased,while that of chromones increased.The relative contents of sesquiterpenes in cold drill,cold drill + salt,fire drill and fire drill + salt treatments were 88.67%,75.34%,56.72% and 54.31%,and the relative contents of chromones were 8.74%,20.96%,27.05% and 43.24%,respectively.③ The contents of agarotetrol and 2-(2-phenylethyl) chromone of cold drills + salt were 1.08 and 0.14 mg/g,which were 2.92 and 13.18 times higher than those of cold drill, respectively.The contents of agarotetrol and 2-(2-phenylethyl) chromone of fire drill + salt were 2.93 and 0.68 mg/g and they were 2.93 and 9.90 times higher than those of fire drill,respectively.【Conclusion】Salt stress significantly increased the contents of agarwood extracts,increased the types of sesquiterpenoids and chromone in fire drill treatments,and induced the production of agarotetrol and 2-(2-phenylethyl) chromone. |
| Key words: agarwood salt stress agarwood formation method agarotetrol 2-(2-phenylethyl) chromone |
