| 摘要: |
| 【目的】优化马尾松松针-绣球菌双向液体发酵条件,并探究绣球菌菌丝体多糖的抗氧化活性，为绣球菌菌丝体的高效生产提供参考。【方法】采用双向液体发酵法,即在绣球菌发酵培养基中添加适量马尾松松针粉末，通过单因素试验和正交试验探究马尾松松针添加量（0.50，0.75，1.00，1.25，1.50 g/L）、发酵时间（12，16，20，24，28，32 d）、绣球菌接种量（25，50，75，100，125，150 mL/L）、pH（4，5，6，7，8）对绣球菌菌丝体生物量的影响，优化双向液体发酵条件。以未添加马尾松松针粉的处理为对照组，提取菌丝体多糖，采用红外光谱对菌丝体的多糖结构进行分析。测定菌丝体多糖对ABTS自由基和羟基自由基的清除率，分析其抗氧化活性。【结果】单因素试验结果显示，当马尾松松针粉末添加量为1.25 g/L、发酵时间为24 d、接种量为100 mL/L、pH为5时，绣球菌菌丝体生物量均较高。正交试验结果显示，马尾松松针 绣球菌双向液体发酵的最优条件为：马尾松松针粉末添加量1.25 g/L、发酵时间24 d、pH为4、接种量125 mL/L，在此条件下绣球菌菌丝体生物量可达到(11.16±0.22) g/L。与对照组相比，双向液体发酵绣球菌菌丝体生物量提高了约162.10%。红外光谱分析结果表明，马尾松松针-绣球菌双向液体法发酵得到的菌丝体多糖的红外主要吸收峰与对照组一致。抗氧化试验结果显示，绣球菌菌丝体多糖对ABTS自由基、羟基自由基均具有较好的清除能力。【结论】得到了马尾松松针-绣球菌双向液体发酵产绣球菌菌丝体的最佳条件，此条件下得到的菌丝体多糖具有良好的抗氧化活性。 |
| 关键词: 绣球菌 马尾松松针 双向液体发酵 菌丝体多糖 抗氧化活性 |
| DOI： |
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| 基金项目:西藏自治区科学技术厅重点研发及转化项目“特色食用菌种质资源创新研究与菌种繁育关键技术集成示范”（XZ202001ZY0041N） |
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| Optimization of bi-direction liquid fermentation conditions of Sparasis crispa mycelium and its polysaccharide antioxidant activity |
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REN Yaqian,ZHUANG Mengying,LIU Xingjian,et al
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| Abstract: |
| 【Objective】The optimal conditions of bi-directional liquid fermentation of Sparasis crispa were explored under Pinus massoniana needles and its mycelium polysaccharides antioxidant activity was studied to provide a reference for efficient production of Sparasis crispa mycelium.【Method】Bi-directional liquid fermentation by adding Pinus massoniana needles powder to fermentation medium was exercised.The single factor test and orthogonal experiment were adopted to examine the effect of Pinus massoniana needles powder addition (0.5,0.75,1.00,1.25,1.50 g/L),fermentation time (12,16,20,24,28 and 32 d),inoculum quantity(25,50,75,100,125 and 150 mL/L),and pH (4,5,6,7,8) on biomass of Sparasis crispa mycelium.Subsequently,mycelium polysaccharides were extracted using the case without Pinus massoniana needles powder as control.Infrared spectroscopy was used to analyze the structure feature of mycelium polysaccharide,and its antioxidant activity was evaluated by ABTS,hydroxyl radical scavenging rates.【Result】The single factor test showed that the mycelium biomass of Sparasis crispa was quite high under conditions of Pinus massoniana needles powder 1.25 g/L,fermentation duration 24 d,inoculum size 100 mL/L and pH 5.The orthogonal test results showed that the optimum bi-direction liquid fermentation conditions were 1.25 g/L Pinus massoniana needles powder,24 d fermentation duration,pH 4 and 125 mL/L inoculum quantity.Under these conditions,the mycelium biomass of Sparasis crispa was (11.16±0.22) g/L,which was 162.10% higher than that of the control group.Infrared analysis indicated the principal absorption peaks of mycelium polysaccharide accorded with those of experimental control.The antioxidant experiment showed that Sparasis crispa mycelium polysaccharides could effectively help scavenge ABTS and hydroxyl radicals.【Conclusion】The optimal conditions for Sparasis crispa mycelium production were obtained by the bi-direction liquid fermentation with Pinus massoniana needles powder,and its mycelium polysaccharide showed good antioxidant activity. |
| Key words: Sparasis crispa Pinus massoniana needles bi-directional liquid fermentation mycelium polysaccharide antioxidant activity |
