| 摘要: |
| 【目的】探索欧拉型藏羊解偶联蛋白2（UCP2）和解偶联蛋白3（UCP3）基因的多态性及其与生长性状的关联性，为运用分子标记辅助育种方法提高欧拉型藏羊的生长性状提供理论依据。【方法】以239头成年欧拉型藏羊为试验对象，采用PCR产物直接测序技术检测UCP2和UCP3基因的遗传多态性，并将其与体质量、体高、体斜长和胸围生长性状进行关联性分析。【结果】在欧拉型藏羊UCP2基因上筛选出2个SNPs位点，分别为g.52130414 C>T和g.52130584 G>A，其中g.52130414 C>T位点产生TT、CT和CC 3种基因型，g.52130584 G>A位点产生AA、AG和GG 3种基因型；在UCP3基因上筛选出1个SNP位点g.52157335 C>T，产生CC、CT和TT 3种基因型。基因多态性分析表明，3个SNPs位点均为错义突变位点，属于中度多态，且均符合Hard-Weinberg平衡适应性检验（P>0.05）。关联分析结果显示，UCP2基因g.52130414 C>T位点TT基因型欧拉型藏羊体质量显著高于CT基因型(P<0.05)；g.52130584 G>A位点AA基因型欧拉型藏羊体质量和体斜长均极显著高于AG和GG基因型(P<0.01),AA和GG基因型欧拉型藏羊胸围均显著高于AG基因型（P<0.05）；UCP3基因g.52157335 C>T位点CC基因型欧拉型藏羊体质量显著高于CT基因型(P<0.05)，TT基因型欧拉型藏羊胸围显著高于CT基因型(P<0.05)。3个多态位点基因型组合分析发现，5个发生频率较高的双倍型中，D5型欧拉型藏羊体质量极显著高于D1、D2、D3型(P<0.01)，体高显著高于D3型(P<0.05)，体斜长显著高于D1、D3、D4型(P<0.05)且极显著高于D2型(P<0.01)。【结论】UCP2和UCP3基因多态性与欧拉型藏羊的部分生长性状显著相关，UCP2和UCP3基因可作为欧拉型藏羊生长性状选育的候选基因。D5双倍型为优势基因型，可在选育工作中优先考虑。 |
| 关键词: 欧拉型藏羊 UCP2 UCP3 基因多态性 生长性状 关联分析 |
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| 基金项目:国家重点研发计划子项目（2018YFD0502403）；中国农业科学院创新工程项目（CAAS-ASTIP-2014-LIHPS-01）；甘肃省草食畜产业技术体系项目（CAR-008） |
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| Association of UCP2 and UCP3 gene polymorphisms and growth traits in Oula-type Tibetan sheep |
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TAN Yizhou,DINGKAO Renqing,CHU Min,et al
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| Abstract: |
| 【Objective】This study explored the polymorphisms of uncoupling protein 2 (UCP2) and uncoupling protein 3 (UCP3) genes and their association with growth traits in Oula-type Tibetan sheep to provide basis for future use of molecular marker-assisted breeding in improving growth traits.【Method】A total of 239 adult Oula-type Tibetan sheep were selected,PCR product direct sequencing was used to detect the genetic polymorphism of UCP2 and UCP3 genes,and the correlations with body weight,body height,body slanting length and chest circumference were analyzed.【Result】Two SNPs loci of g.52130414 C>T and g.52130584 G>A were screened on UCP2 gene of Oula-type Tibetan sheep,and g.52130414 C>T produced three genotypes of TT,CT and CC and g.52130584 G>A produced AA,AG and GG.One SNP site on UCP3 was identified as g.52157335 C>T,which produced three genotypes of CC,CT and TT.The polymorphism analysis showed that the three SNPs sites were missense mutation sites,in moderate polymorphism and conforming to the Hard Weinberg balance (P>0.05).Association analysis showed that body weight of sheep with UCP2 gene g.52130414 C>T locus TT genotype was significantly higher than that of CT genotype (P<0.05),and body weight and body length of g.52130584 G>A AA genotype were significantly higher than those of AG and GG genotypes (P<0.01).Chest circumference of AA and GG genotypes was significantly higher than that of AG genotypes (P<0.05).The weight of sheep with UCP3 gene g.52157335 C>T CC genotype was significantly higher than that of CT genotype (P<0.05),and chest circumference of TT genotype was significantly higher than that of CT genotype (P<0.05).Genotype combination analysis of three polymorphic loci found that the body weight of D5 type was significantly higher than that of D1,D2 and D3 types (P<0.01).Body height of D5 was significantly higher than that of D3 type (P<0.05).Body oblique length of D5 type was also very significantly higher than that of D2 type (P<0.01),and significantly higher than those of D1,D3 and D4 types (P<0.05).【Conclusion】The polymorphisms of UCP2 and UCP3 genes were related to partial growth traits of Oula-type Tibetan sheep,and UCP2 and UCP3 genes could be used as candidate genes for growth traits selection.D5 diplotype was the dominant genotype and could be considered preferentially in breeding. |
| Key words: Oula-type Tibetan sheep UCP2 UCP3 gene polymorphisms growth traits association analysis |
