| 摘要: |
| 【目的】探究刺梨总三萜对免疫抑制小鼠及小鼠RAW264.7巨噬细胞的影响，综合评价刺梨总三萜的免疫活性。【方法】制备刺梨总三萜（TR），给SPF级健康昆明小鼠腹腔注射环磷酰胺（CTX）构建免疫抑制模型，灌胃给予高（200 mg/kg）、中（100 mg/kg）、低（50 mg/kg）剂量刺梨总三萜，每天1次，连续灌胃14 d，试验同时设置空白对照组（不注射CTX，灌胃生理盐水）、模型组（灌胃生理盐水）、阳性对照组（灌胃3 mg/kg香菇多糖片），采集血样及胸腺和脾脏，测算全血白细胞数、胸腺和脾脏指数、血清细胞因子（IL-2、IL-4、IL-6和TNF-α）水平及酸性磷酸酶（ACP）和乳酸脱氢酶（LDH）活力、脾脏组织丙二醛（MDA）含量和过氧化氢酶（CAT）活力；制作小鼠脾脏组织切片，HE染色后观察。检测0（空白对照组），1.25，2.5，5，10，20 μg/mL（终质量浓度）刺梨总三萜对RAW264.7小鼠巨噬细胞增殖的影响，研究低、中、高剂量刺梨总三萜（终质量浓度分别为1.25，2.5，5 μg/mL）对经脂多糖（LPS）诱导的RAW264.7小鼠巨噬细胞NO分泌量的影响。【结果】与模型组相比，不同剂量刺梨总三萜处理小鼠全血白细胞数量、胸腺和脾脏指数均显著或极显著升高，血清中的IL-2、IL-4、IL-6和TNF-α水平大多显著或极显著升高，ACP和LDH活力极显著提高（除低、中剂量组LDH活力外）；脾脏MDA含量极显著降低（P＜0.01），CAT活力极显著升高（P＜0.01）；脾脏组织细胞数量和排列情况均得到了较大改善。1.25~5 μg/mL刺梨总三萜能显著或极显著促进RAW264.7小鼠巨噬细胞增殖，5 μg/mL总三萜可显著抑制LPS诱导的巨噬细胞NO的分泌。【结论】刺梨总三萜能改善由CTX诱导的免疫功能抑制，提高机体抗氧化应激能力，促进小鼠RAW264.7巨噬细胞增殖，抑制LPS诱导的巨噬细胞NO分泌，具有潜在的抗炎免疫活性。 |
| 关键词: 刺梨 总三萜 免疫功能 RAW264.7 抗氧化 |
| DOI： |
| 分类号: |
| 基金项目:贵州省发改委工程中心项目（黔财建［2019］303号）;贵州省科技创新能力建设专项（黔科合服企［2020］4013号）;贵州省教育厅高等学校特色重点实验室项目（黔教合KY字［2020］018号） |
|
| Immunocompetence of total triterpenoids from Rosa roxburghii Tratt fruit |
|
TIAN Qiang,LI Liangqun,PENG Mei,et al
|
| Abstract: |
| 【Objective】This study investigated the effects of total triterpenoids of Rosa roxburghii Tratt (TR) on macrophages of immunosuppressed mice and RAW264.7 mouse and comprehensively evaluated the immunocompetence of TR.【Method】TR were prepared.The immunosuppression model was constructed by intraperitoneal injection of cyclophosphamide (CTX) into SPF-grade healthy Kunming mice,and high (200 mg/kg),medium (100 mg/kg) and low (50 mg/kg) doses of TR were given by gavage once a day for 14 days.The experiment also set up a blank group (with no CTX injection and gavage of saline),a model group (with gavage of saline) and a positive control group (with gavage of 3 mg/kg mushroom polysaccharide tablets).Blood samples,thymus and spleen were collected for determining whole blood leukocyte count,thymus and spleen indexes,serum cytokine (IL-2,IL-4,IL-6 and TNF-α) levels,acid phosphatase (ACP) and lactate dehydrogenase (LDH) activities,spleen tissue malondialdehyde (MDA) levels and catalase (CAT) activity.Spleen sections of mice were prepared and observed after HE staining.The effects of TR levels of 0 (blank group),1.25,2.5,5,10 and 20 μg/mL (final concentration) on the proliferation of macrophages in RAW264.7 mice were investigated.Then,the effects of low,medium and high doses of TR (with final concentrations of 1.25, 2.5 and 5 μg/mL) on NO secretion in macrophages of RAW264.7 mice induced by lipopolysaccharide (LPS) were studied.The experiment was also conducted in the blank group,TR group and model group.【Result】Compared with the model group,the whole blood leukocyte count,thymus and spleen indexes of mice treated with different doses of TR were significantly or very significantly increased.Most serum levels of IL-2,IL-4,IL-6 and TNF-α were significantly or very significantly improved,ACP and LDH viability were very significantly increased (except for LDH viability at low and medium doses),while the spleen MDA level was very significantly decreased (P<0.01).CAT activity was significantly increased (P<0.01).The number and arrangement of splenic tissue cells were greatly improved.TR at levels of 1.25-5 μg/mL significantly or every significantly promoted macrophage proliferation in RAW264.7 mice, while 5 μg/mL of TR significantly inhibited LPS-induced NO secretion by macrophages.【Conclusion】TR can improve CTX-induced immune function suppression and enhance the resistance to oxidative stress,which can promote macrophage proliferation in RAW264.7 mice.It also can significantly inhibit LPS-induced NO secretion by macrophages,showing potential anti-inflammatory immune activity. |
| Key words: Rosa rocburghii Tratt total triterpenoid immune function RAW264.7 antioxidant |
