| 摘要: |
| 【目的】探讨甘胆口服液按推荐剂量用药对牛呼吸道疾病（bovine respiratory disease,BRD）的治疗效果及对血清急性期蛋白（acute phase protein，APP）浓度和氧化应激指标的影响，为其在牛临床上的推广应用提供依据。【方法】选择40头自然发病的BRD犊牛,随机分成2组，分别为受试药物组（甘胆口服液0.2 mL/kg）、对照药物组（麻杏石甘散0.75 g/kg），并增加健康犊牛12头作为空白对照组，按推荐剂量1次/d，连用7 d，分别于试验第0，3，5，7天记录犊牛的一般临床症状,并依据BRD评分表进行打分；在试验第0，7天随机抽取各试验组犊牛12头颈静脉采血，测定血清APP（结合珠蛋白（HP）、淀粉样蛋白A（SAA）、C-反应蛋白（CRP））浓度和氧化应激指标（丙二醛（MDA）、还原型谷胱甘肽（GSH）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC））水平。【结果】药物组试验犊牛第0天证候积分均≥5分，符合试验要求。用药第3，5，7天临床症状积分均不同程度降低（P<0.05）。在第5天时积分均<5分，第7天积分减少到最小值。麻杏石甘散组犊牛在第3天时的BRD治愈率高于甘胆口服液组（P>0.05），但随着治疗时间的延长，甘胆口服液治愈率与麻杏石甘散相近，用药5 d后，两药物组治愈率均达到60%以上。BRD组犊牛第0天血清HP、SAA、MDA水平显著高于健康对照组（P<0.05），GSH、T-AOC水平及SOD、CAT活性均不同程度低于健康对照组，但差异不显著（P>0.05）。经过药物治疗后，BRD组犊牛第7天血清HP、SAA、MDA水平显著下降，GSH水平和SOD活性显著上升（P<0.05）。【结论】甘胆口服液能明显改善BRD犊牛临床症状，有效缓解患BRD犊牛氧化应激状态，降低血清急性期蛋白水平。联合应用急性期蛋白和氧化应激指标可以对BRD犊牛进行早期诊断及疗效监测。 |
| 关键词: 牛呼吸道疾病 甘胆口服液 急性期蛋白 氧化应激指标 |
| DOI： |
| 分类号: |
| 基金项目:四川省科技厅科技计划项目（2019YJ0650,2019YJ0671）；北京生泰尔科技股份有限公司资助项目（002H2001） |
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| Effects of Gandan oral liquid on serum acute protein and oxidative stress biomarkers in calves with respiratory diseases |
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CHEN Jiubing,MA Li,CHEN Yating,et al
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| Abstract: |
| 【Objective】This study explored the therapeutic effect of Gandan oral liquid at recommended dose on bovine respiratory disease (BRD) and the effect on serum acute phase protein (APP) concentration and oxidative stress biomarkers to provide basis for clinical promotion and application of cattle.【Method】A total of 40 calves with naturally occurring BRD were divided into the test drug group (Gandan oral liquid 0.2 mL/kg) and the control drug group (Maxingshigansan 0.75 g/kg) randomly.The drugs were given at the recommended dose once per day for 7 days,using 12 healthy calves as the blank control.The general clinical symptoms of all calves were recorded on days 0,3,5 and 7,and scores were given according to the BRD score table.On days 0 and 7,12 calves from each group were randomly selected to collect blood from jugular vein for determination of serum APP (HP,SAA and CRP) and antioxidant indexes (MDA,GSH,CAT,SOD and T-AOC).【Result】The syndrome scores of calves in the drug group were all greater than or equaled to 5, which met the test requirements.The clinical symptom scores of calves in each BRD group decreased in different degrees 3,5 and 7 days after treatment (P<0.05).The scores were all less than 5 on day 5,and they continued to drop to the minimum on day 7.The Maxingshigansan group had higher curing rate than the Gandan oral liquid group on day 3 (P>0.05).The curing rates got closer with the extension of treatment time,and all reached more than 60% on day 5.The levels of serum HP,SAA and MDA in BRD group were significantly higher than those in healthy control group on day 0 (P<0.05),while GSH level as well as T-AOC,SOD and CAT activities were lower without significant differences (P>0.05).After drug treatment,the levels of serum HP,SAA and MDA in calves decreased significantly on day 7,while the levels of GSH and SOD increased significantly (P<0.05).【Conclusion】The Gandan oral liquid significantly improved clinical symptoms of BRD calves by effectively relieving oxidative stress and reducing the level of serum acute phase protein.The combined application of acute phase proteins and oxidative stress biomarkers can be used for early diagnosis and efficacy monitoring of BRD calves. |
| Key words: bovine respiratory disease Gandan oral liquid acute phase protein oxidative stress index |
