| 摘要: |
| 【目的】研究携带大麦黄矮病毒（barley yellow dwarf virus,BYDV）蚜虫取食对小麦防御基因表达的影响，初步探明BYDV-介体蚜虫-小麦三者的互作机制，为揭示BYDV流行和蚜虫大发生的原因提供理论依据。【方法】以BYDV的主流株系GAV为测试病毒，以其传播介体麦长管蚜和麦二叉蚜为试虫，设置无蚜虫取食小麦（CK）、无毒麦长管蚜取食小麦（Mock-Sa）、无毒麦二叉蚜取食小麦（Mock-Sg）、感染BYDV-GAV麦长管蚜取食小麦（BYDV-Sa）和感染BYDV GAV麦二叉蚜取食小麦（BYDV-Sg）5个处理，于蚜虫取食2 h（蚜虫达到稳定取食所需时间），26，50，74和98 h时，取叶片样品检测茉莉酸防御途径关键基因脂氧合酶基因（LOX）和丙二烯氧化物合成酶基因（AOS）及水杨酸防御途径关键基因病程相关基因非表达子1（NPR1）和苯丙氨酸解氨酶基因（PAL）的表达水平。【结果】与无蚜虫取食对照相比，麦蚜取食能显著提高小麦体内LOX的表达水平，对NPR1的表达水平无显著影响，而对AOS和PAL表达水平的影响因麦蚜种类或处理时间的不同而变化。与无毒蚜虫取食相比，携带BYDV-GAV蚜虫取食总体上能降低小麦体内LOX、AOS、NPR1和PAL的表达水平。【结论】蚜虫取食能提高小麦茉莉酸途径的防御作用，而BYDV-GAV能显著降低小麦茉莉酸和水杨酸途径的防御作用。 |
| 关键词: 大麦黄矮病毒 麦蚜 茉莉酸途径 水杨酸途径 小麦防御基因 |
| DOI： |
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| 基金项目:陕西省科学技术研究发展计划项目（2014K01-07-03） |
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| Effect of feeding by barley yellow dwarf virus infected aphids on defense gene expression in wheat |
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KANG Juxia,LAN Wenxue,YANG Lin
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| Abstract: |
| 【Objective】The effect of feeding by barley yellow dwarf virus (BYDV) infected aphids on expression of defense genes in wheat were studied and the interaction between BYDV,aphids and wheat were explored to provide basis for revealing the cause of BYDV prevalence and aphid occurrence.【Method】Using the wheat vector aphids Sitobion avenae and Schizaphis graminum as test insects and GAV strain of BYDV as test virus,this study included five treatments of wheat without aphid feeding (CK),wheat fed by Sitobion avenae without BYDV (Mock-Sa),wheat fed by Schizaphis graminum without BYDV (Mock-Sg),wheat fed by Sitobion avenae with BYDV (BYDV-Sa and wheat fed by Schizaphis graminum with BYDV (BYDV-Sg).Samples were collected 2 h,26 h,50 h,74 h and 98 h after eating by aphids to determine expression levels of genes LOX (lipoxygenase) and AOS (allene oxide synthetase) in jasmonic acid (JA) synthetic way and genes PAL (phenylalanine ammonia lyase) and NPR1 (nonexpresser of pathogenesis-related genes 1) in salicylic acid (SA) synthetic.【Result】The expression level of LOX was significantly increased in wheat fed by non-virus aphids as compared with no-aphid feeding,while expression level of NPR1 showed no significant difference.The effects on expression levels of AOS and PAL were different among different treatment types and times.Compared with wheat fed by non-virus aphid,expression levels of LOX,AOS,PAL and NPR1 in wheat fed by BYDV-GAV aphid were significantly decreased.【Conclusion】 Aphid feeding can improve defense of JA pathway in wheat,while BYDV-GAV can significantly reduce defense of JA and SA signal pathways in wheat. |
| Key words: barley yellow dwarf virus wheat aphids jasmonic acid pathway salicylic acid pathway defense gene in wheat |
