| 摘要: |
| 【目的】研究甜玉米根系相关性状的QTL，为甜玉米耐密、抗倒伏和抗逆育种提供理论依据。【方法】应用复合区间作图法，以甜玉米组合T49×T56的F2为作图群体，测定F2∶3家系的根长、根干质量和节根层数，并进行QTL定位。【结果】甜玉米遗传连锁图谱包含153个SSR位点，图谱全长1 199.1 cM，平均间距7.83 cM。检测到2个与根长相关的QTL位于第2、8染色体上，贡献率分别为19.70%和18.20%；5个与根干质量相关的QTL位于第3、4、8染色体上，单个QTL可解释5.28%~17.24%的表型变异；3个与节根层数相关的QTL位于第3、8染色体上，单个QTL贡献率为9.38%~21.13%。第8染色体检测到1个同时控制根长和根干质量的QTL位于bin8.02 区域，且与1个bin8.02-8.03区域控制节根层数的QTL紧密连锁，可分别解释18.20%，17.24%和21.13%的表型变异；1个同时控制根干质量和节根层数的QTL位于bin8.03区域，贡献率分别为17.13%和18.82%。【结论】第8染色体上在同一区域内出现控制不同性状的QTL。育种实践中，可利用根长、根干质量和根层数3个性状共同检测到的主效QTL及QTL富集区，进行分子标记辅助选择和遗传改良。 |
| 关键词: 甜玉米 玉米根系 QTL定位 |
| DOI： |
| 分类号: |
| 基金项目:广东省教育厅特色创新项目（2016KTSCX061）；广东省重点领域研发计划项目（2018B020202013）；广东省农业厅种业提升项目（粤财农［2014］492号）；广州市特色作物种质资源研究与利用重点实验室项目（201902020077） |
|
| QTL mapping of root related traits in sweet corn |
|
LIU Pengfei,ZHOU Fuliang,CHEN Qingchun,et al
|
| Abstract: |
| 【Objective】The aim of this study was to study QTL mapping of root related traits in sweet corn to help better breed varieties with tolerance,lodging resistance and stress resistance.【Method】The root length,root dry weight and number of nodal root layers of F2∶3 family from F2 generation of the T49×T56 cross were measured and QTLs for related traits were mapped by composite interval mapping method.【Result】The linkage map contained 153 SSR markers,covering 1 199.1 cM of maize genome with an average distance of 7.83 cM.Two QTLs related to root length were located on chromosomes 2 and 8,explaining 19.70% and 18.20% of phenotypic variation.Five QTLs related to root dry weight were located on chromosomes 3,4 and 8,and a single QTL could explain 5.28%－17.24% of phenotypic variation.Three QTLs related to number of nodal root layers were located on chromosomes 3 and 8,and a single QTL can explain 9.38%－21.13% of phenotypic variation.A QTL simultaneously controlling root length and root dry weight was found on chromosome bin8.02 and it was closely linked to a QTL controlling number of nodal root layers on chromosome bin8.02-8.03.They could explain 18.20%，17.24% and 21.13% phenotypic variations,respectively.A QTL simultaneously controlling root dry weight and number of nodal root layers was detected on chromosome bin8.03,explaining 17.13% and 18.82% of phenotypic variations.【Conclusion】The QTLs controlling different traits were found in the same region on chromosome 8.The main QTLs and the rich and overlapping regions of QTLs co-detected by three traits can be carried out using molecular marker assisted selection and genetic improvement in breeding practice. |
| Key words: sweet corn corn root QTL location |
