| 摘要: |
| 【目的】探讨外源NO对低温胁迫下伏毛铁棒锤种子萌发及幼苗生理特性的影响，为提高伏毛铁棒锤幼苗抗寒性提供理论依据。【方法】以伏毛铁棒锤种子和幼苗为材料，采用不同浓度（0(CK)，50，100，150，200，250，300 μmol/L）的外源NO供体SNP溶液浸种，分别在15（对照），8，4 ℃下培养萌发，统计各处理的萌发率、萌发势和活力指数。在15 ℃下萌发伏毛铁棒锤幼苗，叶片喷施不同浓度（50，100，150，200，250，300 μmol/L）的SNP溶液，以喷施蒸馏水为对照，于4 ℃下进行低温胁迫，通过测定超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性，以及丙二醛（MDA）、过氧化氢（H2O2）、可溶性糖、游离脯氨酸及叶绿素（a＋b）含量，分析SNP对伏毛铁棒锤幼苗抗寒性的影响。【结果】与15 ℃处理相比，8和4 ℃低温处理下伏毛铁棒锤种子的萌发率、萌发势及活力指数均显著降低；施加SNP溶液后，随着SNP溶液浓度的提高，2个低温胁迫处理（8和4 ℃）伏毛铁棒锤种子的萌发率、萌发势及活力指数均呈先升高后降低的趋势，且均在200 μmol/L SNP时达到最大值。4 ℃低温处理下，与对照相比，200 μmol/L SNP溶液处理伏毛铁棒锤幼苗体内SOD、POD、CAT活性，可溶性糖、游离脯氨酸及叶绿素（a＋b）含量均显著提高，MDA、H2O2含量均显著降低（P<0.05）。【结论】施加200 μmol/L SNP溶液可缓解低温下伏毛铁棒锤种子及幼苗受到的冷害，提高种子及幼苗的抗寒能力。 |
| 关键词: 低温胁迫 伏毛铁棒锤 外源NO 抗寒性 |
| DOI： |
| 分类号: |
| 基金项目:中医药公共卫生服务补助专项“全国中药资源普查项目”（财社（2017）66号） |
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| Effects of exogenous NO on seed germination and seedling physiological characteristics of Aconitum flavum Hand.-Mazz under low temperature stress |
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RU Gang,CHEN Xuelin,YU Wenhui,et al
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| Abstract: |
| 【Objective】This study explored the effect of adding exogenous NO on seed germination and seedling physiological characteristics of Aconitum flavum Hand.-Mazz under low temperature stress to provide basis for improving cold resistance of Aconitum flavum Hand.-Maz seedlings.【Method】Seeds of Aconitum flavum Hand.-Mazz were soaked with different concentrations of exogenous NO donor SNP solutions (0,50,100,150,200,250 and 300 μmol/L),germination was conducted at 15 (control),8 and 4 ℃,and germination rate,germination potential and vitality index were counted.Seedlings were germinated at 15 ℃ and leaves were sprayed with different concentrations of SNP solutions using distilled water as control.Then,seedlings were placed at 4 ℃ for low temperature stress and activities of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT) as well as contents of malondialdehyde (MDA),hydrogen peroxide (H2O2),soluble sugar,free proline and chlorophyll (a+b) were measured to analyze effect of SNP on cold resistance of seedlings.【Result】Compared with the treatment of 15 ℃,germination rate,germination potential and vigor index of seeds in treatments of 8 and 4 ℃ were significantly reduced.As solution concentration increased,germination rate,germination potential and vigor index of seeds in two treatments of low temperature increased first followed by decrease with maximum values at 200 μmol/L SNP.At low temperature treatment of 4 ℃,treatment of 200 μmol/L SNP solution increased activities of SOD,POD and CAT, significantly increased contents of soluble sugar,free proline and chlorophyll(a+b),while significantly decreased contents of MDA and H2O2(P<0.05).【Conclusion】The application of 200 μmol/L SNP solution can alleviate cold damage to seeds and seedlings of low-lying Aconitum flavum Hand.-Mazz at low temperature and improve cold resistance of seeds and seedlings. |
| Key words: low temperature stress Aconitum flavum Hand.-Mazz exogenous NO mitigation effect |
