| 摘要: |
| 【目的】研究高蛋白饲粮对肉鸡肾氧化损伤及黄嘌呤氧化酶（xanthine oxidase,XOD）的影响。【方法】选取120羽20日龄健康白羽肉杂鸡，随机分为4组，每组5个重复，每个重复6羽。对照组饲喂基础饲粮（蛋白质含190 g/kg），试验组饲喂在基础饲粮中添加豆粕使粗蛋白含量分别达到220，250，280 g/kg的饲粮。试验期为14 d。于试验第0，7和14天，分别从每组随机选取5羽鸡经翅下静脉采血，分离血清，用于肾功能（血清钙（Ca）、肌酐（CREA）、尿素氮（BUN）、尿酸（UA）、血清磷（P）、镁（Mg）含量）、脂质过氧化（血清丙二醛（MDA）、一氧化氮（NO）、羟自由基（·OH）含量和总抗氧力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性）及XOD活性检测。试验结束时，每组选5羽鸡进行解剖，取肾脏组织，用于检测XOD基因mRNA的表达量。【结果】1）与对照组相比，各高蛋白饲粮组肉鸡血清Ca、CREA、BUN、UA含量显著或极显著上升（P＜0.05或P＜0.01），血清P、Mg含量变化不显著（P＞0.05）。2）与对照组相比，各高蛋白饲粮组肉鸡血清MDA、NO、·OH含量显著或极显著升高（P＜0.05或P＜0.01），T-AOC、GSH-Px、SOD活性显著或极显著降低（P＜0.05或P＜0.01）。3）与对照组相比，各高蛋白饲粮组XOD活性显著升高（P＜0.05），肾组织XOD mRNA表达量显著增加（P＜0.05）。【结论】高蛋白饲粮可改变肉鸡肾功能与脂质过氧化，导致血清XOD活性以及肾组织XOD mRNA表达量升高，导致鸡肾脏损伤。 |
| 关键词: 高蛋白饲粮 肉鸡 肾功能 脂质过氧化 黄嘌呤氧化酶 |
| DOI： |
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| 基金项目:国家重点研发计划项目(2016YFD0501205)；安徽省重点研究与开发计划项目(1804a07020135) |
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| Effects of high protein diet on renal oxidative damage and mRNA expression of xanthine oxidase gene in broilers |
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ZHAO Jie,XU Jingru,CAO Li，et al
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| Abstract: |
| 【Objective】The aim of this experiment was to study the effects of high protein diet on renal function, lipid peroxidation and xanthine oxidase (XOD) in broilers.【Method】One hundred and twenty white-feathered broilers at the age of 20-day-old were randomly divided into four groups with 5 repeats each group and 6 feathers each repeat.The control group was fed on a basic diet with protein content of 190 g/kg,while the experimental groups were fed on a diet with soybean meal added to the basal diet for crude protein contents of 220,250 and 280 g/kg,respectively.The trial was conducted for 14 days.In days 0,7 and 14,5 feathers from each group were randomly selected and blood samples were collected from the subwing vein to separate serum.Then,renal function (serum calcium (Ca),creatinine (CREA),urea nitrogen (BUN),uric acid (UA),serum phosphorus (P) and magnesium (Mg) contents),lipid peroxidation (serum malondialdehyde (MDA),nitric oxide (NO),hydroxyl free radical (·OH) contents and total antioxidant (T-AOC),glutathione peroxidase (GSH Px) and superoxide dismutase (SOD) activities) and XOD activity were determined.At the end of the experiment,5 feathers were randomly selected from each group for dissecting,and kidney tissues were taken to detect expression of XOD gene mRNA.【Result 1) Compared with the control group, serum contents of Ca,CREA,BUN and UA in each high protein diet group had significant or extremely significant increase (P＜0.05 or P＜0.01),while the changes in P and Mg were insignificant (P＞0.05).2) Compared with the control group,contents of MDA,NO and ·OH in serum of high protein diet groups were increased significantly or extremely significantly (P＜0.05 or P＜0.01),while activities of T-AOC,GSH-PX and SOD were significantly or extremely significantly decreased (P<0.05 or P< 0.01). 3) Compared with the control group,XOD activity in each high protein diet group was significantly higher (P＜0.05),and expression level of XOD mRNA in renal tissue of high protein group was significantly increased (P＜0.05).【Conclusion】High protein diet can change renal function and lipid peroxidation of broilers,leading to increase of XOD activity and expression of XOD mRNA in renal tissue and causing renal damage to chickens. |
| Key words: high protein diet broiler renal function lipid peroxidation xanthine oxidase |
