| 摘要: |
| 【目的】研究吉林克雷伯氏菌2N3菌株对噻吩磺隆的降解特性及其对土壤的修复效果，为2N3菌株实际应用奠定基础。【方法】利用HPLC和振荡培养法，研究噻吩磺隆初始质量浓度、接菌量、温度、pH、NaCl体积分数以及土壤是否灭菌情况下，克雷伯氏菌2N3对噻吩磺隆的降解效果。【结果】在无机盐液体培养基中，2N3降解噻吩磺隆的最优条件为：噻吩磺隆初始质量浓度50 mg/L，2N3接菌量为5%（体积分数），pH 7.0，NaCl体积分数为0.3%。在此条件下于30 ℃、150 r/min恒温振荡培养24 h，噻吩磺隆的降解率可达90.41%。在土壤中，2N3对噻吩磺隆最高降解率可达96.03%。在未灭菌土壤中，2N3对噻吩磺隆的降解速率较灭菌土壤快，说明其能协助土壤微生物共同降解噻吩磺隆。【结论】吉林克雷伯氏菌2N3菌株能有效降解噻吩磺隆，并对噻吩磺隆污染土壤有较好的修复效果。 |
| 关键词: 噻吩磺隆 克雷伯氏菌 降解特性 土壤修复 |
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| 基金项目:国家重点研发计划项目（2016YFD0200203）；吉林省科技厅科技攻关项目（20190301063NY） |
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| Characteristics of Klebsiella jilinsis 2N3 in thifensulfuron-methyl degradation and soil remediation |
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LIN Yang,PAN Zequn,ZHANG Jinpeng,et al
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| Abstract: |
| 【Objective】The characteristics of bacteria Klebsiella jilinsis 2N3 in degrading thifensulfuron-methyl and its remediation effect on soil were studied to improve its practical application.【Method】The effects of 2N3 on degradation of thifensulfuron methyl were studied by HPLC and shaking culture methods with different conditions of initial mass concentration,inoculation amount,temperature,pH,NaCl volume fraction,and whether soil was sterilized.【Result】In an inorganic salt liquid medium,the optimal conditions for thifensulfuron-methyl degradation by 2N3 included initial mass concentration of thifensulfuron-methyl 50 mg/L,2N3 inoculation amount 5%(volume fraction),pH 7.0,NaCl volume fraction 0.3%,30 ℃,150 r/min constant temperature shaking and culture for 24 h.The obtained degradation rate of thifensulfuron-methyl was 90.41%.The highest degradation rate of thifensulfuron methyl in soil was 96.03%.The degradation rate of thifensulfuron methyl in unsterilized soil was higher than that in sterilized soil,indicating soil microorganisms were assisted to degrade thifensulfuron-methyl together.【Conclusion】Bacteria Klebsiella jilinsis 2N3 can effectively degrade thifensulfuron-methyl and has good repair effects on thifensulfuron-methyl contaminated soil. |
| Key words: thifensulfuron methyl Klebsiella jilinsis degradation characteristics soil remediation |
