| 摘要: |
| 【目的】研究外源硅对肉桂酸（trans-cinnamic acid,CA）模拟自毒胁迫下黄瓜种子萌发和生理特性的影响，为黄瓜自毒作用的缓解提供参考。【方法】以‘新春四号’黄瓜为试材，添加2 mmol/L肉桂酸模拟黄瓜种子自毒胁迫，再施用外源硅（Na2SiO3·9H2O，AR），通过人工气候箱培养，以不添加外源硅为对照（CK），测定不同处理黄瓜种子的发芽率、胚根长、鲜质量、丙二醛、可溶性糖和淀粉含量以及淀粉酶、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性。【结果】与CK相比，2 mmol/L CA处理显著降低了黄瓜种子发芽率、胚根长和鲜质量，较CK分别减少了92.00%，91.46%和36.77%，显著增加了黄瓜种子中可溶性糖、淀粉和丙二醛（MDA）含量，降低了黄瓜种子中α-淀粉酶、β-淀粉酶和总淀粉酶活性以及SOD、POD和CAT的活性，从而严重抑制了黄瓜种子的萌发。在2 mmol/L CA胁迫下外源添加3 mmol/L硅酸钠能显著增加黄瓜种子的发芽率、胚根长及鲜质量，分别较CA处理增加了91.48%，84.19%和30.31%，降低了黄瓜种子中可溶性糖、淀粉和MDA含量，提高了α-淀粉酶、β-淀粉酶和总淀粉酶以及SOD、POD和CAT的活性，进而减轻了活性氧对细胞膜的损伤，促进了碳水化合物的转化速率。【结论】添加外源硅对肉桂酸模拟自毒胁迫的黄瓜种子有明显的缓解作用。 |
| 关键词: 黄瓜 肉桂酸 自毒胁迫 种子萌发 生理特性 硅 |
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| 基金项目:国家自然科学基金项目（31660584）；甘肃省科技重大专项（17ZD2NA015）；甘肃省自然科学基金项目（1610RJZA098）；甘肃农业大学盛彤笙科技创新基金项目（GSAU-STS-1638） |
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| Effects of silicon on physiological characteristics of cucumber seed germination under self-toxic stress |
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LIU Fanhong,Lǚ Jian,YU Jihua,et al
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| Abstract: |
| 【Objective】This study investigated the effect of exogenous silicon on physiological characteristics of cucumber seed germination under simulated self toxic stress using cinnamic acid (trans-cinnamic acid,CA) to provide reference for alleviating the self-toxic effect.【Method】‘Xinchun 4’ seeds were treated with 2 mmol/L cinnamic acid to simulate self-toxic stress,and exogenous silicon (Na2SiO3·9H2O，AR) was applied under artificial climate box culturing taking no exogenous silicon treatment as the control (CK).The germination rate, radicle length,fresh quality,malondialdehyde and available nitrogen of cucumber seeds,contents of soluble sugar and starch,and activities of amylase,superoxide dismutase,peroxidase and catalase under different treatments were measured.【Result】Compared with CK,2 mmol/L CA significantly reduced germination rate,radicle length and fresh weight of cucumber seeds by 92.00%,91.46% and 36.77%，and significantly increased contents of soluble sugar and starch.It also decreased total activities of amylase,β-amylase and α-amylase,and significantly increased accumulation of malondialdehyde (MDA).Under the stress of 2 mmol/L CA,germination rate,radicle length and fresh weight of cucumber seeds were significantly increased by adding 3 mmol/L sodium silicate,and they were 91.48%,84.19% and 30.31% higher than that of CA.Contents of soluble sugar and starch were decreased and activities of total amylase,β-amylase and α-amylase were increased.Activities of SOD,POD and CAT were significantly increased,MDA content was decreased,damage of active oxygen on cell membrane was reduced,and conversion rate of carbohydrate was promoted.【Conclusion】The addition of exogenous silicon had a significant alleviating effect on cucumber seeds under cinnamic acid simulated self-toxic stress. |
| Key words: cucumber cinnamic acid self-toxic stress seed germination physiological characteristics silicon |
