| 摘要: |
| 【目的】分析国内外11份苜蓿种子萌发期的抗旱性，以筛选出适宜内蒙古科尔沁半干旱地区种植的苜蓿品种。【方法】采用15％聚乙二醇6000（PEG-6000）溶液模拟干旱胁迫条件，以蒸馏水为对照（CK），测定11份苜蓿种子萌发期的胚根长、胚芽长，计算胚根长/胚芽长、发芽率、发芽势、发芽指数、活力指数及其抗旱系数等14项指标，通过方差分析筛选差异显著的指标。在主成分分析的基础上，利用加权隶属函数对供试种子的抗旱性进行综合评价。【结果】与CK相比，在15％PEG-6000胁迫下，除骑士T和威神以外，其他苜蓿品种种子的发芽率均降低；除威神以外，其他苜蓿品种种子的活力指数均降低；11份苜蓿种子的发芽势和发芽指数均降低。通过方差分析筛选出了差异显著的9项指标，在主成分分析的基础上，根据加权隶属函数法，综合评价出11份苜蓿种子萌发期抗旱性强弱表现为：农菁8号＞公农1号＞威神＞骑士T＞敖汉苜蓿＞巨能MT3015＞Dryland＞挑战者＞宁苜1号＞32 IQ＞北极熊。【结论】根据种子萌发试验，初步认为农菁8号、公农1号和威神抗旱性较强，更适合内蒙古科尔沁半干旱地区种植；主成分分析结合加权隶属函数法对于苜蓿抗旱性综合性评价具有较好应用效果。 |
| 关键词: 苜蓿 种子萌发 抗旱性 加权隶属函数 半干旱地区 |
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| 基金项目:国家牧草产业技术体系专项（CARS-34）；“十三五”重点研发计划项目“干草低损耗高品质规范化生产及产品加工技术研究与示范”（2017YFD0502103） |
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| Identification and evaluation of drought resistance of alfalfa at germination stage under PEG-6000 stress |
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HAO Junfeng,ZHANG Yuxia,JIA Yushan,et al
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| Abstract: |
| 【Objective】This study analyzed the drought resistance of 11 seeds from domestic and foreign countries to select alfalfa varieties suitable for planting in the semi-arid area of Horqin,Inner Mongolia.【Method】The drought stress condition was simulated by using 15% polyethylene glycol 6000(PEG-6000) solution and distilled water was used as the control (CK).The radicle length and embryo length of each seed during germination period were measured,and 14 indexes including radicle length/embryo length,germination rate,germination potential,germination index and drought resistance coefficient were calculated.Significant differences were tested through analysis of variance.On the basis of principal component analysis,the weighted membership function was used to comprehensively evaluate the drought resistance of tested alfalfa.【Result】Compared with the control,germination rates of materials declined except Knight T and Weishen,and vigor index declined except Weishen.Germination potential and germination index of all materials declined under drought stress.Nine indexes with significant differences were screened by analysis of variance.Based on principal component analysis and weighted membership function method,the drought resistance was in the order of Nongjing No.8>Gongnong No.1>Weishen> Knight T>Aohan alfalfa>GN MT3015>Dryland>Challenger>Ningmu No.1>32 IQ>Polar bear.【Conclusion】Nongjing No.8,Gongnong No.1 and Knight T had strong drought resistance and were more suitable for planting in the semi arid area of Horqin,Inner Mongolia.Principal component analysis combined with weighted membership function method had good application potential for the comprehensive evaluation of drought resistance. |
| Key words: alfalfa seed germination drought resistance weighted membership function semi-arid area |
