| 摘要: |
| 【目的】研究外源5-氨基乙酰丙酸（ALA）对低温胁迫下茶树叶片光合特性的调控效应，为提高茶树抗寒性提供理论依据。【方法】以‘陕茶1号’（耐低温型）和‘金牡丹’（低温敏感型）两个茶树品种为试验对象，于入冬前叶面喷施不同质量浓度（10，30，50 mg/L）的ALA，以喷施清水为对照，分析不同质量浓度外源ALA对茶树叶片内源ALA合成途径下游代谢中间产物、光合气体交换参数及叶绿素荧光参数的影响。【结果】一定质量浓度的外源ALA可以明显提高‘陕茶1号’和‘金牡丹’的内源ALA、原卟啉Ⅸ、Mg 原卟啉Ⅸ、原叶绿素酸酯（pchlide）、叶绿素a和叶绿素b的含量，增强茶树叶片净光合速率（Pn）、胞间二氧化碳浓度（Ci）、气孔导度（Gs）、蒸腾速率（Tr），降低茶树瞬间水分利用率（WUE）和气孔限制值（Ls），提高PSⅡ最大光化学效率（Fv/Fm）和PSⅡ潜在活性（Fv/Fo）。【结论】外源ALA促进了低温胁迫下茶树叶片叶绿素的累积，提高了光合作用能力，增强了茶树抵抗低温的能力，其中50 mg/L ALA明显提高了耐低温型茶树‘陕茶1号’应对低温胁迫的能力，而30 mg/L ALA对缓减低温敏感型茶树‘金牡丹’低温伤害的效果更好。 |
| 关键词: 茶树 5-氨基乙酰丙酸 低温胁迫 光合作用 |
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| 基金项目:陕西省教育厅项目(16JS019)；陕西理工大学博士启动基金项目(SLGQD15-14)；陕南秦巴生物资源综合开发协同创新中心项目（QBXT-Z(Z)-15-2）;陕西省科技厅农业攻关项目（2018NY-126） |
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| Effect of exogenous 5-aminolevulinic acid on photosynthetic characteristics of tea plants under low temperature stress |
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JIANG Wenhua,YAN Fei,FU Jing,et al
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| Abstract: |
| 【Objective】This study explored the mechanism of exogenous ALA to alleviate low temperature stress from the perspective of photosynthetic regulation and investigated the effect of exogenous 5-aminolevulinic acid (ALA) on photosynthetic characteristics of tea plants under low temperature stress.【Method】‘Shaancha 1’ (cold tolerant) and ‘Jinmudan’ (cold susceptible) were treated with different concentrations of ALA (10,30 and 50 mg/L) before winter using water as control.The effects of exogenous ALA on metabolic intermediates of endogenous ALA synthesis pathway,photosynthetic gas exchange parameters and chlorophyll fluorescence parameters in tea leaves were analyzed.【Result】Appropriate concentrations of exogenous ALA increased the contents of endogenous ALA,protoporphyrin Ⅸ,Mg protoporphyrin Ⅸ,pchlide,chlorophyll a and chlorophyll b,and improved net photosynthetic rate (Pn),intercellular carbon dioxide concentration (Ci),stomatal conductance (Gs) and transpiration rate (Tr).The instantaneous water use efficiency (WUE) and stomatal limit value (Ls) were decreased and the maximum photochemical efficiency (Fv/Fm) and PSII potential activity (Fv/Fo) were enhanced.【Conclusion】Exogenous ALA promoted the accumulation of chlorophyll,increased the photosynthetic capacity in tea plants under low temperature stress, and enhanced the ability of tea to resist low temperature.ALA at 50 mg/L significantly improved the cold resistant ability of ‘Shaancha1’ under low temperature stress,while ALA at 30 mg/L was better to relieve the damage caused by low-temperature stress to ‘Jinmudan’. |
| Key words: tea plants 5-aminolevulinic acid low temperature stress photosynthetic fluorescence |
