| 摘要: |
| 【目的】挖掘新的控制水稻抽穗期的QTLs，为水稻花期调控的遗传机理研究和分子育种提供新的基因资源。【方法】以籼稻品种特籼占空间诱变突变体CHA 1和籼稻航恢7号空间诱变突变体H335为亲本进行杂交，构建包含275个株系的重组自交系（RIL）作图群体，利用由两亲本重测序及RIL群体简化基因组测序所构建的高密度遗传图谱，在2个环境下进行水稻抽穗期QTL定位。【结果】经两亲本重测序及RIL群体简化基因组测序，构建了包含2 498个Bin标记的高密度遗传图谱。该图谱覆盖水稻12条染色体，各染色体标记数平均208.17，标记间平均遗传距离为0.95 cM。在2个环境下共定位到4个影响抽穗期的QTLs，分布于第3、第6和第8号染色体，其中qHD-6和qHD-8-1位于前人已报道定位的区域并被克隆，另外2个QTLs（qHD-3和qHD-8-2）尚未见报道，并且qHD-8-2能在2个环境中被重复检测到，贡献率分别为8.30%和10.89%。【结论】通过构建的高密度遗传图谱在2个环境中共检测到4个影响抽穗期的QTLs，其中qHD-3和qHD-8-2是新的抽穗期QTL位点，可用于后续抽穗期调控基因的精细定位及克隆研究，也可用于水稻抽穗期的分子调控机理研究与育种。 |
| 关键词: 水稻 重组自交系 抽穗期 QTL定位 Bin标记 |
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| 基金项目:国家现代农业产业技术体系建设专项(CARS-01-12)；国家重点研发计划项目(2017YFD0100104)；广东省自然科学基金项目（2018A0303130340） |
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| Mapping new rice heading date QTLs based on high-density genetic map |
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LI Dongxiu,YANG Jing,SUN Kai,et al
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| Abstract: |
| 【Objective】This study excavated new QTLs controlling heading date of rice to provide new genetic resources for genetic mechanism research and molecular breeding.【Method】The mapping population of 275 recombinant inbred lines (RIL) was obtained by crossing indica rice varieties mutant CHA-1 and indica rice space mutagenesis mutant H335 of Hanghui 7.QTL mapping at heading date of rice was carried out in two environments using high density genetic map established by whole genome sequencing (WGS) of both parents and RIL population genotyping-by-sequencing (GBS).【Result】A high density genetic map containing 2 498 Bin markers was constructed by WGS of parents and GBS of RIL population.The map covered 12 chromosomes of rice,with an average number of markers of 208.17 and an average genetic distance of 0.95 cM between markers.A total of 4 QTLs associated with heading date were detected on chromosomes 3,6,and 8.The qHD-6 and qHD-8-1 were overlapped with known loci and cloned,while qHD-3 and qHD-8-2 were newly reported.In addition,qHD-8-2 could be detected in both environments with the contribution rates of 8.3% and 10.89%,respectively.【Conclusion】Four QTLs affected heading date were detected in the two environments by constructing a high density genetic map.Among them,qHD-3 and qHD-8-2 were new QTL loci at heading date,and they can be used for fine mapping and gene cloning,as well as molecular regulation mechanism at heading date. |
| Key words: rice recombinant inbred lines heading date QTL mapping Bin marker |
