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夏枯草农艺性状与其有效成分相关性研究
郭巧英1, 梁宗锁2, 赵宏光,等3
1.西北农林科技大学 草业与草原学院;2.浙江理工大学 生命科学与医药学院;3.陕西天士力植物药业有限责任公司
摘要:
【目的】研究夏枯草主要农艺性状与其有效成分含量之间的相关性,为夏枯草的品质改良和品种选育提供参考。【方法】以航天诱变夏枯草种植第6代(SP6)的Ⅰ(分枝多)、Ⅱ(大果穗)、Ⅲ(株型分散)、Ⅳ(二级侧枝)、Ⅴ(早熟)、Ⅵ(晚熟)和Ⅶ(株型紧凑)及CK(地面对照)为材料,通过五点取样法选定单株,测定其农艺性状及有效成分含量,并对测定结果进行数据分析、差异性分析、相关性分析和主成分分析。【结果】夏枯草大果穗性状与植株干鲜质量、分枝数、果穗数、冠幅呈显著正相关关系,按照该性状选育有利于得到高产、大穗株系。株高、冠幅、分枝数、果穗数、果穗粗、果穗长、果穗干质量各农艺性状之间存在显著正相关关系,相互依赖性高。其中,分枝数与冠幅、果穗干质量、果穗长、果穗粗、果穗数、植株鲜干质量呈极显著正相关关系,它可能是对夏枯草产量影响最大的因子。各株系的有效成分变异系数均较大,存在丰富的变异,且各株系有效成分含量之间未见明显规律。株系Ⅵ(晚熟)的总酚、总黄酮含量最高,各有效成分之间存在显著相关性。迷迭香酸、咖啡酸、总酚、总黄酮4者之间存在极显著正相关关系;阿魏酸与迷迭香酸、咖啡酸、总酚、总黄酮之间存在极显著负相关关系;齐墩果酸与其他有效成分相关性不显著;各有效成分含量与各果穗指标相关性均不显著。株系Ⅵ(晚熟)和株系Ⅶ(株型紧凑)有效成分含量最高,适合作为优质株系进行筛选。【结论】夏枯草植株越嫩,其总酚和总黄酮含量越高;根据果穗数、分枝数、冠幅性状指标进行选育有利于获得高产优质果穗,其中分枝数可能是影响果穗产量的最大因子。
关键词:  夏枯草  农艺性状  有效成分  品种选育
DOI:
分类号:
基金项目:国家科技部“十三五”科技支撑计划项目(2015BAC01B03-01);浙江理工大学科研启动基金项目(17052059-Y)
Correlation analysis of main agronomic traits with active constituents of Prunella vulgaris
GUO Qiaoying,LIANG Zongsuo,ZHAO Hongguang,et al
Abstract:
【Objective】In order to provide reference for quality improvement and variety selection of Prunella vulgaris,the correlation between agronomic traits and contents of active ingredients of P.vulgaris was analyzed.【Method】The individual plants of the sixth generations of P.vulgaris (SP6) were selected by five point sampling method.The measured plants included Ⅰ (more branches),Ⅱ (large ear),Ⅲ (disperse plants),Ⅳ (secondary side branch),Ⅴ (early ripening),Ⅵ (late ripening),Ⅶ (compact plants),and control (CK).Data analysis,differential analysis,correlation analysis and principal component analysis were conducted to the measured traits and contents.【Result】There was significant positive correlations between the large ear traits and dry weight,number of branches,number of spikes and crown width of plants.Choosing large ear was beneficial to high yield and large-ear lines.There was significant positive correlation in agronomic traits among plant height,crown width,number of branches,number of ears,ear diameter,ear length and dry weight of ear with high interdependence.The number of branches had very significant correlation with other agronomic traits,and it was the most influential factor on yield of Prunella vulgaris.The variation coefficients of effective components of each strain were large,indicating abundant variations.There was no obvious regularity between active components of each strain.The average contents of rosmarinic acid,caffeic acid,total phenol and total flavonoids were higher than that of previous three generations.The total contents of total phenol and total flavonoids in strain Ⅵ (late ripening) were the highest.There was significant correlation between contents of each active ingredient.There was significant positive correlation between rosmarinic acid,caffeic acid,total phenol and total flavonoids.There was significant negative correlation between ferulic acid and rosmarinic acid,caffeic acid,total phenol and total flavonoids.The correlation between fruit acid and other active ingredients was insignificant.The correlation between content of each active ingredient and indicator characteristics of each ear was insignificant.【Conclusion】Younger plants had higher contents of total phenols and total flavonoids.The selection based on number of spikes,branches and crown traits was conducive to obtain high yield and high quality ear.Number of branches was the largest factor affecting yield of P.vulgaris.
Key words:  Prunella vulgaris  agronomic traits  active ingredients  breeding of variety