| 摘要: |
| 【目的】对玉米中长链酰基辅酶A合成酶1（ZmLACS1）基因及其蛋白磷酸化位点进行鉴定，为研究玉米脂肪酸的降解及其利用机制奠定基础。【方法】通过结构域分析查找质谱鉴定到蛋白中的长链酰基辅酶A合成酶1，通过系统发育树分析判断玉米中长链酰基辅酶A合成酶1与拟南芥超家族中长链酰基辅酶A合成酶的同源性，通过质谱分析鉴定该蛋白的磷酸化修饰位点。通过3D建模模拟长链酰基辅酶A合成酶1的结构以及磷酸化位点所在位置。【结果】玉米长链酰基辅酶A合成酶1（ZmLACS1）与拟南芥中的长链酰基辅酶A合成酶1（AtLACS1）具有高度同源性；通过质谱分析鉴定到3个该蛋白的磷酸化位点分别为S360、Y365和S366，同时鉴定到的磷酸化位点位于长链酰基辅酶A合成酶的保守结构域（AMP-binding domain）中，3个磷酸化位点位置一致，位于两个α螺旋中间，处于蛋白结构的关键位置。【结论】磷酸化修饰可能是调节长链酰基辅酶A合成酶1功能的关键性方式之一，ZmLACS1可能与AtLACS1在功能上具有相似性。 |
| 关键词: 玉米 长链酰基辅酶A合成酶 磷酸化修饰 蛋白结构 |
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| 基金项目:国家自然科学基金项目（31770207）；科技部“十二五”农村领域国家科技计划项目（2015BAD15B0503） |
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| Identification of long chain acyl-CoA synthase 1 and detection of its phosphorylation sites in Zea mays |
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CHEN Ying,WANG Xiaofeng,CHEN Shaolin
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| Abstract: |
| 【Objective】This study identified the long chain acyl-CoA synthase 1 in Zea mays and detected its phosphorylation sites to provide basis for studying the mechanism of fatty acid degradation and utilization in Zea mays.【Method】Long chain acyl CoA synthase 1 was detected by domain analysis and phylogenetic analysis was used to estimate its similarity with long chain acyl-CoA synthases in Arabidopsis superfamily.Mass spectrum was used to identify phosphorylation sites.The 3D structure of long chain acyl-CoA synthase 1 was modeled and the location of phosphorylation sites was confirmed.【Result】Long chain acyl-CoA synthase 1 in Zea mays and Arabidopsis thaliana was highly homologous.Phosphorylation sites of S360,Y365 and S366 were detected by mass spectrum and they were located in the conserved domain of long chain acyl-CoA synthase family (AMP-binding domain).The location of identified phosphorylation sites was consistent and they were in an important location between two α-spirals.【Conclusion】Phosphorylation may be an important way for regulating the function of long chain acyl-CoA synthase 1 and it is speculated that it had similar function in Zea mays and Arabidopsis thaliana. |
| Key words: Zea mays long chain acyl-CoA synthase 1 phosphorylation protein structure |
