| 摘要: |
| 【目的】分离陕西秦岭丹江流域土壤中的黏细菌，收集其代谢产物，对代谢产物成分和活性进行分析。【方法】采用大肠杆菌诱导法和滤纸片诱导法分离从丹江流域采集的18份土壤样品中的黏细菌，用摇瓶发酵得到其代谢产物，采用枯草芽孢杆菌、金黄色葡萄球菌、藤黄微球菌和大肠杆菌作为指示菌对代谢产物进行平板抑菌试验；对4种指示菌抑制活性明显菌株的代谢产物成分进行HPLC和薄层色谱(TLC)分析，采用细胞凋亡法测试了代谢产物组分对HepG2肝癌细胞的毒性。【结果】共分离纯化出23株黏细菌，包括黏球菌属（Myxococcus）15株，珊瑚球菌属（Corallococcus）3株，孢囊杆菌属（Cystobacter）1株，原囊菌属（Archangium）3株，软骨霉状菌属（Chondromyces）1株。抑菌试验表明15号菌株的2号代谢产物对4种指示菌均有不同程度的抑制作用。对HPLC得到的15号菌株2号代谢产物的5号组分进行研究，结果表明，其能明显增加HepG2细胞凋亡。【结论】秦岭丹江流域土样中的黏细菌具有一定的多样性，得到1株活性菌株，其代谢产物具有抑菌活性和抗肿瘤细胞活性。 |
| 关键词: 黏细菌 代谢产物 生物活性 丹江流域 |
| DOI： |
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| 基金项目:陕西省科技厅重点研发计划项目(2017NY-116,2017NY-139);陕西省科学院应用基础研究专项(2015K-12) |
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| Isolation and metabolites of myxobacteria from the Danjiang River in Qinling Mountains |
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WANG Jun,LI Hongduo,ZHANG Kun,et al
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| Abstract: |
| 【Objective】Myxobacteria were isolated from soils of the Danjiang River in Qinling Mountains,Shaanxi,and their metabolites were collected to analyze the composition and activities.【Method】Myxobacteria were isolated from 18 soil samples collected from Danjiang River by the living Escherichia coli and filter paper methods.The metabolites were obtained by fermentation.Biological activity experiments were carried out by the test bacteria of B.subtilis,S.aureus,M.luteus and E.coli.The strains with inhibitory activities against 4 indicator bacteria were analyzed and their metabolites were separated by HPLC and thin layer chromatography (TLC).The toxicity effect of metabolites to HepG2 on human hepatocellular carcinoma cells line was tested by apoptosis assay in vitro.【Result】A total of 23 strains were isolated including Myxococcus (15 strains),Corallococcus (3 strains),Cystobacter (1 strains),Archangium (3 strains) and Chondromyces (1 strains).Antibacterial experiments showed that metabolite No 2 of strain 15 had inhibition against 4 tested indicator bacteria.Component No 5 of metabolite No 2 of strain 15 obtained by HPLC was further studied,and it can significantly induce apoptosis of HepG2 cells.【Conclusion】The diversity of myxobacteria from soil samples of Danjiang River was rich,and the metabolites of an obtained strain had antibacterial and antitumor activities. |
| Key words: myxobacteria metabolites biological activities Danjiang River |
