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3个梅花品种幼苗耐盐性综合评价
杨佳鑫1, 李庆卫2, 郭子燕,等2
1.北京林业大学 园林学院,花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,教育部林木花卉育种实验室;2.北京林业大学 园林学院,花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,教育部林木花卉育种实验室
摘要:
【目的】研究不同梅花(Prunus mume)品种幼苗的耐盐性,为梅花盐碱土地区推广应用提供依据。【方法】以香雪宫粉、丰后、美人3个梅花品种1年生自根苗为试材,设置土壤NaCl含量分别为0(对照),3,6,9,12,15 g/kg, 处理35 d后观测不同含量NaCl胁迫下3个梅花品种的盐害指数、盐害率以及叶片的形态、生长和生理指标的变化;采用主成分分析法和隶属函数法相结合的方法,对3个梅花品种耐盐性进行综合评价。【结果】(1)随着NaCl胁迫含量的升高,3个梅花品种盐害指数和盐害率均逐渐增大,叶长、叶宽、株高、新梢长度生长量总体呈下降趋势。(2)与对照相比,NaCl胁迫后3个梅花品种相对电导率,游离脯氨酸、可溶性糖、可溶性蛋白含量和SOD、POD活性总体呈上升趋势;叶片组织含水量总体呈下降趋势。随着NaCl含量的升高,香雪宫粉MDA含量呈先升后降趋势,丰后和美人MDA含量呈升-降-升的趋势。(3)3个梅花品种耐盐能力从强到弱依次为香雪宫粉>丰后>美人。【结论】香雪宫粉、丰后、美人3个梅花品种幼苗的耐盐性有差异,其耐盐阈值分别是6.96,6.53,6.09 g/kg,耐盐能力从强到弱依次为香雪宫粉>丰后>美人。
关键词:  梅花品种  NaCl胁迫  盐害指数  耐盐性评价
DOI:
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基金项目:科学研究与研究生培养共建科研项目“北京实验室 北京城乡节约型绿地营建技术与功能型植物材料高效繁育”(2016GJ-03)
Comprehensive evaluation on salt tolerance of three Prunus mume varieties
YANG Jiaxin,LI Qingwei,GUO Ziyan,et al
Abstract:
【Objective】This study investigated salt resistance of different P. mume varieties to provide theoretical basis for promoting the application of P. mume in saline soil.【Method】One-year-old own-rooted seedlings of P. mume Xiangxue Gongfen,Fenghou,and Meiren were tested for 35 d under salt stresses of 0 (control),3,6,9,12,and 15 g/kg.The salt injury index,salt injury rate,and form,growth and physiology indicators of leaves were observed to investigate the morphological and physiological responses to salt stress.Principal component analysis and membership function method were used to comprehensively evaluated the slat tolerance.【Result】(1) With the increase of soil NaCl content,the salt injury index and salt injury rate of three P. mume varieties gradually increased,while the increments of leaf length,leaf width,stem length,and new shoots length decreased.(2) Compare with the control,the relative conductivity,contents of free protein, soluble sugar and soluble protein content,and SOD and POD activities increased,while leaf tissue moisture decreased.With the increase of soil NaCl content,MDA content of Xiangxue Gongfen increased firstly and then decreased.MDA contents of Fenghou and Meiren increased firstly and then decreased and increased in the end.(3) The overall salt tolerance was in the order of Xiangxue Gongfen>Fenghou>Meiren.【Conclusion】There were differences in salt resistance among three P. mume varieties.The salt tolerance thresholds of Xiangxue Gongfen,Fenghou,and Meiren were in the decreasing order of 6.96,6.53,and 6.09.The overall salt tolerance was in the order of Xiangxue Gongfen>Fenghou>Meiren.
Key words:  Prunus mume variety  NaCl stress  salt injury index  salt tolerance evaluation