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不同葡萄品种叶绿素荧光参数的高温响应及其耐热性评价
吴久赟1,2, 廉苇佳1, 刘志刚,等1
1.新疆农业科学院 吐鲁番农业科学研究所;2.石河子大学 农学院
摘要:
【目的】研究19个葡萄品种叶绿素荧光参数对高温的响应表现,并对其耐热性进行分析和评价,为吐鲁番耐热葡萄品种的选育及其耐热机理研究提供依据。【方法】以新疆农业科学院吐鲁番农业科学研究所葡萄资源圃的19个葡萄品种为试验材料,在吐鲁番的自然高温条件下,利用快速荧光测定仪(Handy-PEA)测定葡萄叶片的F0Fm、Fv/F0Fv/Fm、PIABS、DI0/CS0ΦPSⅡ、φE0ETR、qP等10个主要荧光参数,分析不同品种葡萄叶绿素荧光参数在高温下的表现,采用模糊隶属函数法结合有序样本最优分割聚类法对其耐热性进行评价。【结果】19个葡萄品种的叶绿素荧光参数在高温下均受显著影响,导致光能转化效率降低,其耐热性综合评价结果为美人指>矢富罗莎>金田蜜>红地球>金田玫瑰>藤稔>红旗特早玫瑰>无核白>和田黄>巨玫瑰>克瑞森无核>贝达>巨峰>木纳格>乍娜(绯红)>维多利亚>里扎马特>无核白鸡心>水晶无核。采用有序样本最优分割聚类法分析发现,美人指、矢富罗莎、金田蜜、红地球、金田玫瑰、藤稔属耐热性较强的葡萄品种。【结论】筛选出了适宜在吐鲁番地区种植的耐热性较强的6个葡萄品种。
关键词:  葡萄品种  叶绿素荧光参数  高温响应  耐热性
DOI:
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基金项目:新疆农业科学院青年基金项目“吐鲁番葡萄的耐热性分析评价”(xjnkq-2017012);新疆维吾尔自治区公益性科研院所基本科研业务费资助项目“新疆葡萄资源收集、整理与评价”(KYGY2016130);农业部农业基础性长期性科技工作项目“国家作物种质资源数据中心观测监测”(ZX01S3221)
High temperature response of chlorophyll fluorescence parameters and heat tolerance evaluation of different grape cultivars
WU Jiuyun,LIAN Weijia,LIU Zhigang,et al
Abstract:
【Objective】This study investigated high temperature response of chlorophyll fluorescence of different grape cultivars and evaluated heat tolerance for 19 grape cultivars to provide basis for breeding heat tolerant grapes and studying heat tolerant mechanisms of grapes in Turpan.【Method】A total of 19 grape varieties from Turpan Research Institute of Xinjiang Academy of Agricultural Sciences were selected.The chlorophyll fluorescence characteristic parameters including F0,Fm,Fv/F0,Fv/Fm,PIABS,DI0/CS0,ΦPSⅡ,φE0,ETR and qP in leaves of grape were determined by rapid fluorescence detector (Handy-PEA) under the natural high temperature condition in Turpan.The responses of these parameters to high temperature were analyzed and compared among different grape cultivars.The fuzzy membership function analysis method and the sample optimal segmentation clustering method were then used to evaluate the heat tolerance.【Result】The chlorophyll fluorescence parameters of all 19 cultivars were affected significantly by high temperature with decreased light energy transformation rate.The comprehensive scores were in the order of ‘Manicure finger’>‘Yatomi Rosa’>‘Jintianmi’>‘Red Globe’>‘Jintian Meigui’>‘Fuji minori’>‘Hongqi Tezaomeigui’>‘Thompson Seedless’>‘Hetianhuang’>‘Jumeigui’>‘Crimson Seedless’>‘Beta’>‘Kyoho’>‘Munage’>‘Cardinal’>‘Victoria’>‘Rizamat’>‘Centennial Seedless’>‘Shuijing Wuhe’.The sample optimal segmentation clustering method with classification standard showed that ‘Manicure finger’,‘Yatomi Rosa’,‘Jintianmi’,‘Red Globe’,‘Jintian Meigui’ and ‘Fujiminori’ had strong heat tolerance.【Conclusion】Six grape varieties with strong heat tolerance suitable for cultivation in Turpan area were selected.
Key words:  grape cultivars  chlorophyll fluorescence parameter  high temperature response  heat tolerance