| 摘要: |
| 【目的】探索独脚金内酯对干旱环境下‘赤霞珠’幼苗生长的影响。【方法】以酿酒葡萄‘赤霞珠’实生苗为材料，叶面喷施0.2 mg/L独脚金内酯的人工合成类似物GR24，以喷施蒸馏水为对照，3 d后进行自然干旱处理（停止浇水），采用烘干称重法每天检测土壤相对含水量，分别在自然干旱第4 （水分适宜期）、8 （轻度胁迫期）、12（中度胁迫期）和18（重度胁迫期）天采样，研究独脚金内酯处理后干旱胁迫对葡萄幼苗生长、根系、生物量、叶绿素含量和细胞超微结构的影响。【结果】干旱胁迫过程中，与对照相比，独脚金内酯处理组‘赤霞珠’幼苗叶片变黄、萎蔫的程度均有所减轻，幼苗生长状态更好，株高和茎粗升高，地上部和地下部干鲜质量、根冠比均极显著增加；独脚金内酯处理组‘赤霞珠’幼苗根长、根系平均直径、根系表面积和根体积均极显著提高，根尖数和根分枝数增多；独脚金内酯处理组‘赤霞珠’幼苗叶片相对含水量呈降低趋势，但变化曲线较对照平缓，叶片电导率和相对水分亏缺呈上升趋势，但变化过程也较对照组平缓；随干旱胁迫时间的延长，各处理‘赤霞珠’幼苗叶绿素a、叶绿素b及总叶绿素含量均呈降低趋势，但独脚金内酯处理组幼苗叶绿素含量的降幅较对照组小。细胞超微结构观察表明，与对照相比，独脚金内酯处理组‘赤霞珠’幼苗叶肉细胞和根尖细胞结构更完整。【结论】喷施独脚金内酯对干旱胁迫下‘赤霞珠’幼苗的生长发育有促进效应。 |
| 关键词: 葡萄 干旱胁迫 独脚金内酯 赤霞珠 幼苗生理特性 |
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| 基金项目:宁夏自治区十三五重点研发项目“优质特色酿酒葡萄生产精准水肥调控研究”（2016BZ0602）；国家现代农业（葡萄）产业技术体系建设专项（CARS-29-ZP-6） |
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| Effects of strigolactones on growth of Cabernet Sauvignon seedlings under drought stress |
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LI Runyu,MIN Zhuo,FANG Yulin,et al
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| Abstract: |
| 【Objective】The objective of this study was to explore the effect of strigolactone on physiological characteristics of Cabernet Sauvignon seedlings under drought conditions.【Method】Strigolactone analogues (GR24,0.2 mg/L) were foliar sprayed on leaves of Cabernet Sauvignon seedlings,and distilled water was sprayed as control.After three days,Cabernet Sauvignon seedlings were stopped watering under stimulant natural drought conditions.Soil relative water content was measured daily by drying weighing method.The physiological characteristics of grape seedlings including seedling growth characteristics,root properties,biomass,chlorophyll content,and cell ultrastructure were studied on the 4 day (suitable water content period),8 day (mild stress period),12 day (moderate water period) and 18 day (severe water period) after natural drought.【Result】Compared to the control group, during the drought stress process,Cabernet Sauvignon seedlings in treatment group grew better.With GR24 treatment,the severity of younger leaves yellowing and wilting was lessened,and plant height and stem thickness were increased.Both plant fresh dry mass of aboveground part or underground part and the root-shoot ratio of underground part were significantly promoted.Under drought stress,root length,root average diameter and root surface area or volume of grape seedlings of treatment group increased significantly, and the numbers of root branches and tips were markedly higher than those of the control group.The relative water contents of seedling leaves of treatment group showed a slow decreasing trend compared with the control.GR24 treatment could alleviate the increase of electrical conductivities of seedling leaves and seeding relative water deficit.With increased drought stress,the contents of chlorophyll a,chlorophyll b and total chlorophyll showed decreasing trend,and the decrease was less than the control.The structure of mesophyll cells and root tip cells in treatment group was more integrated than the control.【Conclusion】Foliar application of strigolactone could alleviate the adverse effects of drought stress on grape seedlings. |
| Key words: grape drought stress strigolactones Cabernet Sauvignon seedling physiological characteristics |
