| 摘要: |
| 【目的】建立唐棣离体植株组织培养快繁体系，并对该体系进行优化。【方法】以唐棣带腋芽的茎段为外植体获得无菌苗，再以无菌试管苗为材料，以MS为基础培养基，采用正交试验研究6 BA(0.5,1.0,1.5 mg/L）、NAA(0.02,0.04,0.06 mg/L）、IBA(0.05,0.10,0.15 mg/L)配比对唐棣组培苗扩繁的影响，并研究不同基本培养基(WPM,1/4MS,1/8MS)和NAA(0.1,0.3 mg/L)、IBA(0.5,1.0 mg/L)配比对唐棣无菌苗生根的影响；再以已生根的试管苗为材料，研究不同开瓶时间(12,18,24,36,48 h)、生根时间(15,20,25,30 d)、基质配比 (国产泥炭、国产水苔、腐殖质体积比分别为1∶1∶1，1∶3∶1，1∶5∶1和1∶4∶2)对炼苗移栽成活率和生长状况的影响。【结果】最适合唐棣带腋芽茎段的消毒方法是体积分数75%酒精消毒30 s，再用1 g/L HgCl2消毒8 min，芽萌发率达到17.3%；最适合组培苗增殖的激素组合为MS+0.5 mg/L 6-BA+0.02 mg/L NAA+0.05 mg/L IBA，增殖系数达到6.28；最适合无菌苗生根的培养基为1/4MS+0.5 mg/L IBA，生根率达到100%；在生根培养25 d后开瓶适应24 h，然后再进行炼苗，最适合炼苗的基质配比为V(国产泥炭)∶V(国产水苔)∶V(腐殖质)=1∶5∶1，试管苗移栽成活率达到70.00%。【结论】建立并优化了唐棣组织培养体系，明显提高了无菌苗的增殖数量、生长健康指数及生根率，并且炼苗后移栽成活率也显著提高。 |
| 关键词: 唐棣 组织培养 生根培养 炼苗移栽 |
| DOI： |
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| 基金项目:国家林业局林业公益性行业科研专项（201204308） |
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| Establishment of the Amelanchier sinica regeneration system and optimization |
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HE Zhiyu,FU Xuening,YIN Pengxian,et al
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| Abstract: |
| 【Objective】This study aimed to establish and optimize a tissue culture system for Amelanchier sinica.In the a large number of seedlings can be bred.【Method】Taking A. sinica embryo tissue of somatic embryo callus (embryoid) as test materials,using the method of in vitro culture,the orthogonal experiment was used to study the influence of the proportion of 6-BA（0.5,1.0,1.5 mg/L）,NAA（0.02,0.04,0.06 mg/L） and IBA（0.05,0.10,0.15 mg/L） on the propagation of tissue culture seedlings of A.sinica,and the influence of the proportion of different basic media（WPM,1/4MS,1/8MS）,NAA（0.1,0.3 mg/L） and IBA（0.5,1.0 mg/L） on the sterile rooting seedlings of A.sinica was studied.Then the test tube seedlings of rooting were used to study the influence of different bottle opening time（12,18,24,36,48 h）,rooting time（15,20,25,30 d） and the proportion of matrix（the volume ratio of domestic peat,domestic water moss,and humus is 1∶1∶1,1∶3∶1,1∶5∶1,1∶4∶2） on the survival rate of transplanted seedlings.【Result】The best disinfection method was 75% ethanol for 30 s and 1 g/L HgCl2 for 8 min.Optimal shoot proliferation occurred on MS medium with 0.5 mg/L 6-BA and 0.02 mg/L NAA and 0.05 mg/L IBA.The best rooting was 1/4MS with 0.5 mg/L IBA,with rooting rating of 100%.The optimal matrix ratio was V (humus)∶V(sphagna)∶V(peat)=1∶5∶1,watered three times a day with a diminishing cycle of 10 days,after opening a bottle of 24 hours,rooting culture lasts 44 days and the survival rate was 70.00% after 30 days.【Conclusion】The established and optimized tissue culture system for A.sinica improved reproduction rate and the survival rate of transplanting of tissue cultured seedlings increased obviously. |
| Key words: Amelanchier sinica tissue culture rooting culture hardening seedling |
