| 摘要: |
| 【目的】分析甘蓝型油菜骨干亲本的遗传多样性，揭示参试材料的遗传差异。【方法】利用筛选到的8对SSR引物和12对SRAP引物，对30份甘蓝型油菜骨干亲本材料及1份芥菜型油菜和1份白菜型油菜材料进行遗传多样性分析，结合聚类分析、主成分分析、群体结构分析以及分子方差分析揭示参试材料之间的遗传多样性。【结果】在参试材料中，8对SSR引物共扩增出49条多态性条带，多态性比率为96.08%，平均每对引物扩增6.1条，平均多态性信息含量（PIC）为0.43，遗传距离均值0.34；12对SRAP引物共扩增出77条多态性条带，多态性比率为98.72%，平均每对引物扩增6.4条，PIC为0.59，遗传距离均值0.46。UPGMA聚类分析表明，在遗传相似系数0.65处，大部分参试材料被分为两大类，第Ⅰ类包括19份材料，其中14份为恢复系，5份为保持系；第Ⅱ类包括9份材料，均为保持系。主成分分析、群体结构分析的结果与聚类分析结果基本一致。分子方差分析结果显示，参试甘蓝型油菜群体内变异系数为95.46%，群体间变异系数仅为4.54%。保持系与恢复系材料间差异为2.03%。【结论】参试甘蓝型油菜骨干亲本间存在一定的遗传差异，恢复系和保持系群体内部遗传变异大于群体间的遗传变异。 |
| 关键词: 油菜 骨干亲本 遗传多样性 SSR标记 SRAP标记 |
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| 基金项目:西北农林科技大学唐仲英作物育种基金项目（A212021713） |
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| Genetic diversity pattern of elite lines in Brassica napus L.based on SSR and SRAP markers |
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ZANG Shan,ZHANG Yunxiao,GUO Yuan,et al
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| Abstract: |
| 【Objective】The study revealed the genetic diversity of elite lines in Brassica napus L.【Method】Thirty elite lines of B.napus L.,one Brassica juncea L.cultivar and one Brassica campestris L.cultivar were genotyped using 8 pairs of SSR primers and 12 pairs of SRAP primers,and their genetic diversity patterns were analyzed by combination of cluster analysis,principal component analysis,population analysis,and molecular variance analysis.【Result】A total of 49 polymorphic fragments were detected using 8 SSR primer pairs,and the polymorphism rate was 96.08%,with an average number of polymorphic fragments per primer pair of 6.1,the average polymorphism information content of 0.43,and the genetic distance was 0.34.A total of 77 polymorphic fragments were detected using 12 SRAP primer pairs,and the polymorphism rate was 98.72%,with an average number of polymorphic fragments per primer pair of 6.4,the average polymorphism information content of 0.59,and the genetic distance was 0.46.The unweighted pair-group method with arithmetic mean cluster analysis indicated that most accessions could be divided into 2 major clusters with the coefficient cut off value of 0.65.The first cluster consisted of 19 materials,of which 14 were restorer lines and 5 were maintainer lines.The second cluster consisted of 9 materials,and all were maintainer.The results of principal component analysis and population structure analysis were similar to that of cluster analysis.Molecular variance analysis revealed that the genetic variation was 95.46% within B.napus restorers and maintainers and was 4.54% among them.The difference between B.napus maintainers and restorers was 2.03%.【Conclusion】There was certain genetic difference between the parent lines of tested rapeseed,and the genetic variation within the restorers and maintainers was greater than among them. |
| Key words: Brassica napus L. elite line genetic diversity SSR marker SRAP marker |
