| 摘要: |
| 【目的】分离筛选具有抑菌、降解纤维素及抗氧化等功能的亳菊内生菌，为其在农业上的开发应用奠定基础。【方法】以株龄9个月的安徽亳菊为材料，分别取根、茎、叶、花4部分，采用平板培养法从中分离内生菌菌株，并在平板上筛选和检测分离菌株对玉米弯孢菌、黄瓜枯萎菌、小麦赤霉菌、串珠镰刀菌、瓜炭疽菌、茶叶轮斑菌等6种植物病原菌的抑菌活性，测定目标菌株BJF10对纤维素的降解活性和DPPH的清除能力，根据菌体形态特征和ITS序列系统发育树对菌株BJF10进行鉴定。【结果】从亳菊的根、茎、叶、花中共获得了56株内生菌菌株，其中菌株BJF10对6种植物供试病原菌均有较强的拮抗作用，抑菌率均在50％以上；菌株BJF10培养第4天的纤维素降解活性达到最强，培养3 d时羧甲基纤维素酶（CMCase）和滤纸酶（FPase）的活力达到最高,分别为180.32和81.46 U/mL；BJF10的7 d培养上清液对DPPH的清除率可达67.7％；根据形态特征及ITS序列的系统发育分析，将菌株BJF10鉴定为柄孢壳菌属(Zopfiella sp.)。【结论】柄孢壳菌属亳菊内生真菌BJF10菌株具有拮抗植物病原菌、降解纤维素、抗氧化等生物活性。 |
| 关键词: 亳菊 内生菌 抑菌活性 纤维素降解 抗氧化活性 |
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| 基金项目:阜阳市政府与阜阳师范学院横向合作项目（XDXH2016007）；安徽高校省级自然科学研究一般项目（2014KJ018）；阜阳师范学院研究生创新研究计划项目（2017CXJH05，2017CXJH07） |
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| Isolation,identification and biological activity analysis of an endophytic fungus from Chrysanthemum morifolium cv.Boju |
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WANG Guisheng,LU Juan,TANG Juan,et al
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| Abstract: |
| 【Objective】The paper aimed to isolate and screen endophytic fungi with ability of antifungus,antioxidation and cellulose degradation from Chrysanthemum morifolium cv.Boju.It may lay the foundation for further development and application in agriculture.【Method】The 9-month-old Chrysanthemum morifolium cv.Boju was divided into roots,stems,leaves,and flower parts.Antagonism of endophytic fungus BJF10 against Curvularia lunata,Fusarium graminearum,Fusarium moniliforme,Fusarium oxysporum,Colletotrichum lagenarium and Pestalotiopsis theae were evaluated by plate confrontation assay.The CMC enzyme activity,FPA activity and ability of scavenging DPPH were also studied.The strain BJF10 was identified according to the morphological features and ITS sequence analysis.【Result】The endophytic fungus strain BJF10 has strong antagonistic effects on six plant pathogens and the inhibitory rate was above 50%.The strain reached the highest cellulase activity after incubation for four days.The CMC enzyme activity and FPA activity reached 180.32 and 81.46 U/mL,respectively.The DPPH scaveging reached 67.7% by fermentation supernatant of BJF10.The strain BJF10 was identified as Zopfiella sp.based on morphological characteristics and ITS sequence analysis.【Conclusion】The endophytic fungus BJF10 has good ability of resistance to plant pathogens,cellulose degradation,and antioxidation. |
| Key words: Chrysanthemum morifolium cv.Boju endophytes antimicrobial activity cellulose degradation antioxidation activity |
