| 摘要: |
| 【目的】筛选对榆叶梅（Prunus triloba）幼苗生长发育起促进作用的植物生长调节剂种类及质量浓度，为榆叶梅的壮苗提供依据。【方法】以1年生榆叶梅幼苗为试验材料，采用盆栽试验，蘸根施用多效唑（PP333）、生根粉（GGR6）2种药剂，质量浓度均设50，100，150，200 mg/L 4个梯度，分别记为P50、P100、P150、P200和G50、G100、G150、G200处理，以清水处理为对照（CK），测定不同处理榆叶梅生长指标（株高、冠幅、基径）和生理生化指标（叶绿素a（Chl a）、叶绿素b（Chl b）、叶绿素总含量（Chl（a+b））、电子传递效率（ETR）、实际光化学量子效率（ΦPSⅡ）、光化学猝灭（qP）、非光化学猝灭（qN）、最大光化学效率（Fv/Fm）），研究2种药剂对盆栽榆叶梅生长发育的影响,对所测的指标进行相关性分析,并利用隶属函数对不同处理榆叶梅测定指标进行综合评价。【结果】PP333抑制了榆叶梅幼苗株高的生长，当其质量浓度为150 mg/L时，对榆叶梅幼苗株高的抑制程度最大；GGR6促进了榆叶梅幼苗株高的生长，当其质量浓度为100 mg/L时，对榆叶梅幼苗株高的促进程度较大。PP333和GGR6均能明显促进榆叶梅幼苗基径增粗及冠幅的增加。随着PP333和GGR6质量浓度的增加，榆叶梅幼苗的叶绿素含量及叶绿素荧光参数ETR、ΦPSⅡ、qP、Fv/Fm均呈现先增大后减小的趋势，且PP333和GGR6质量浓度分别为150和100 mg/L时达到峰值，均与对照（CK）有显著差异（P<0.05）；qN呈现先减小后增大的趋势。相关分析表明，Chl a、Chl b、Chl（a+b）与叶绿素荧光参数ETR、ΦPSⅡ、qP、Fv/Fm呈现极显著正相关关系（P<0.01），与qN呈现极显著负相关关系（P<0.01）。各处理隶属函数平均值大小顺序为G100> P150> P200> P100>G50>G200>G150>P50>CK。【结论】榆叶梅最佳促进剂为GGR6，且当其质量浓度为100 mg/L时，对榆叶梅幼苗生长发育的综合促进作用最佳。 |
| 关键词: 榆叶梅 植物生长调节剂 叶绿素含量 叶绿素荧光参数 |
| DOI： |
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| 基金项目:国家林业局“948”项目“人工调控荒漠灌丛生态空间构型技术引进”（2015-4-22） |
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| Effects of plant growth regulators on growth and chlorophyll fluorescence parameters of Prunus triloba |
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WEI Yajuan,LIU Zongqi,WANG Ji,et al
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| Abstract: |
| 【Objective】This study selected the plant growth regulators and their concentrations for promoting the growth of Prunus triloba seedlings to provide theoretical basis for breeding strong seedlings.【Method】The annual seedlings of Prunus trilobata were selected for pot experiments.The roots were treated with paclobutrazol (PP333) and rooting powder (GGR6).The mass concentrations were 50,100,150,and 200 mg/L,and the treatments were recorded as P50,P100,P150,P200,G50,G100,G150,and G200 as well as water as control (CK).The growth indexes (plant height,crown width,and base diameter) and physiological and biochemical indicators (chlorophyll a (Chl a),chlorophyll b (Chl b), total chlorophyll (Chl(a+b)),electron transport rate (ETR),actual photochemical efficiency (ΦPSⅡ),photochemical quenching (qP),non photochemical quenching (qN),and maximum photochemical efficiency (Fv/Fm)) were measured.The effects of the two growth regulators were studied on the growth and development of Prunus triloba.The correlation analysis was used to perform measured indicators and the membership function was used to carry out comprehensive evaluation to indicators of different treatments.【Result】The application of 150 mg/L PP333 was the most effective treatment on inhibiting the plant height growth of Prunus triloba seedlings.GGR6 treatment promoted height growth of Prunus triloba seedlings with the best concentration of 100 mg/L.PP333 and GGR6 significantly promoted the growth of base diameter.With the increase of PP333 and GGR6 concentrations,the chlorophyll content and chlorophyll fluorescence parameters including ETR,ΦPSⅡ,qP,and Fv/Fm increased first and then decreased.The peak values were obtained when the concentrations of PP333 and GGR6 were 150 and 100 mg/L,all had significant difference from the control (CK) (P<0.05).The qN showed a trend of decreasing first and increasing afterwards.The correlation analysis showed that Chl a,Chl b and Chl(a+b) were significantly positively correlated with chlorophyll fluorescence parameters ETR,ΦPSⅡ,qP,and Fv/Fm (P<0.01),and significantly negatively correlated with qN (P<0.01).The order of the averaged values of membership functions was G100>P150>P200>P100>G50>G200>G150>P50>CK.【Conclusion】The best accelerating agent for Prunus triloba was GGR6 and the best effect was obtained at the concentration of 100 mg/L. |
| Key words: Prunus triloba plant growth regulators chlorophyll content chlorophyll fluorescence parameters |
