| 摘要: |
| 【目的】研究酵母硒对围产期奶牛代谢的影响，从代谢物变化的角度阐述其在分娩应激状态下的生物学作用。【方法】选择24头体况、胎次、年龄、体质量和预产期相近的健康围产前期奶牛随机分为对照组和试验组，每组各12头，对照组饲喂基础日粮，试验组在基础日粮中添加0.15 g/kg干物质的酵母硒，于产前21 d开始饲喂，分娩后当天停止饲喂。在产前21 d及分娩后6 h内尾静脉采血，使用EDTA抗凝并离心收集血浆。应用UPLC-TOF/MS分析技术,在正负离子模式下对所有血浆样品进行代谢组学分析，数据经预处理后利用多维统计（包括主成分分析（principal component analysis, PCA）和正交偏最小二乘法判别分析（orthogonal partial least squares-discriminant analysis,OPLS-DA)）和单变量（t-检验和差异倍数）分析方法，分析在产前21 d和分娩当天试验组和对照组奶牛血浆中代谢物差异性，以OPLS-DA获得的VIP值>1和t检验中P<0.1为条件筛选差异代谢物。【结果】2组奶牛血浆代谢物在饲喂硒前差异不明显，饲喂硒后有19种差异代谢物。与对照组相比，试验组奶牛在分娩后血浆中L-苯丙氨酸、L-亮氨酸、异亮氨酸、L-谷氨酰胺、L-缬氨酸、酪胺、酮亮氨酸和硫酸吲哚酚显著降低（P<0.05），甜菜碱、7-甲基黄嘌呤、马尿酸、尿囊素和柠檬酸显著升高（P<0.05），L-正亮氨酸、DL-苯丙氨酸、乙二醇单乙醚和氧代己二酸呈降低趋势（0.05<P<0.1），L-肌肽和苯乙酰甘氨酸呈升高趋势（0.05<P<0.1）。【结论】在分娩应激状态下，硒可能通过影响氨基酸、脂类和尿嘌呤衍生物的代谢，发挥着抗应激反应的作用。 |
| 关键词: 酵母硒 奶牛 围产期 代谢组学 分娩应激 |
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| 基金项目:国家自然科学基金地区科学基金项目(31660731)；四川农业大学学科建设双支计划项目（03571537） |
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| Effect of selenium yeast on plasma metabolism of transition dairy cow in parturition stress status |
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LUO Zhengzhong,BAI Lipeng,HUANG Yixin,et al
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| Abstract: |
| 【Objective】The aim of this study was to detect the effect of selenium yeast on metabolism of transition dairy cows and to explain the biological functions of selenium yeast during parturition stress.【Method】A total of 24 transition dairy cows with similar body condition scoring,parity,age and due date were selected and randomly divided into a test group and a control group.The 12 cows in each group were fed on basal diet supplemented with or without 0.15 g selenium yeast per kg dry matter (DM) from 21 days prepartum to postpartum.Plasma from caudal vein was collected 21 d prepartum and 6 h postpartum.Serum samples were subjected to metabolic profiling analysis using ultra performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF/MS) in positive and negative modes.Pretreatment data was performed by multivariate statistical methods including principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) as well as univariate analysis including t-test or fold difference method.The differences of plasma metabolites were analyzed between test group and control group 21 d prepartum and the delivery day.The differential metabolites were selected based on the VIP score (>1) obtained form OPLS-DA model and P<0.1 in t-test.【Result】The plasma metabolites of two groups had no significant difference before feeding selenium,but there were 19 differential metabolites after feeding selenium.Compared with control group,L-phenylalanine,L-leucine,isoleucine,L-glutamine,L-valine,tyramine,leucine,and indoxyl sulfate in test group were significantly reduced (P<0.05),while betaine,7-methylxanthine,hippuric acid,allantoin and citrate were significantly increased (P<0.05）.L-norleucine,DL-phenylalanine,2-ethoxyethanol and 2-oxoadipic acid in test group were lower than that of the control (0.05<P<0.1),while L-creatine and phenylacetylglycine were higher (0.05<P<0.1).【Conclusion】The selenium may play an important role in anti-stress process by regulating the metabolism of aminoacid,lipids and purine derivatives during parturition stress. |
| Key words: selenium yeast dairy cows transition period metabolomics parturition stress |
