| 摘要: |
| 【目的】研究硬毛粗盖孔菌(Funalia trogii)子实体不同生长阶段的生物学特性及脂溶性化学成分的变化，为深入了解其生长过程中内部化学物质的变化规律及开发利用提供基础。【方法】采用徒手切片法对不同生长时期的组织结构进行显微镜观察并记录；通过GC-MS检测硬毛粗盖孔菌不同生长时期的脂溶性成分，并将检测结果进行对比。【结果】在子实体个体发育过程中，表层菌丝首先分化出盖面组织，经过3次从白色蜂窝状结构至浅黄褐色菌毛的转变过程，菌肉逐渐增厚，内部空腔数量增多、总空间增大。灰褐色或浅黄褐色的齿状菌管起源于盖面组织边缘的菌丝组织。待子实层发育成熟后，部分栅栏状细胞层变为普通丝状菌丝，互相靠近交织，在齿状菌管之间相连，最终形成与自然状态下形态、结构相近的子实体个体。GC-MS分析显示，硬毛粗盖孔菌不同生长时期共检测出10种脂溶性化学成分，其中菌球期检测出8种，盖面组织发育完成期全被检测出（10种），子实体形成期检测出8种，3个时期共检测出56个脂溶性化合物。【结论】硬毛粗盖孔菌个体经过盖面组织分化、菌管与子实层分化以及子实层中菌丝变化3个阶段，可以发育形成类自然状态的子实体；且不同生长阶段的脂溶性成分种类和含量都不同。 |
| 关键词: 硬毛粗盖孔菌 生物学特征 GC-MS分析 显微结构 子实体 |
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| 基金项目:教育部长江学者和创新团队发展计划项目（IRT-15R25）；吉林省高等学校高端科技创新平台建设项目 |
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| Biological characteristics and GC-MS analysis of Funalia trogii at different growth periods |
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ZHANG Liang,XIA Jiale,QIAN Danzhu,et al
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| Abstract: |
| 【Objective】This study investigated the biological characteristics and lipsoluble components of Funalia trogii at different growth periods to provide basis for further understanding internal chemical substances and its development and utilization.【Method】Macroscopic observation and description of the growth and development of rough F. trogii were conducted and the tissue structure of different growth stages was observed by microscope and recorded by the hand section method.The lipid soluble components were detected by GC-MS during the growth stages and the test results were compared.【Result】In the development process of basidiocarp,the surface hyphae differentiated first from the surface tissue.After three times from white honeycomb structure to light brown fungus,context gradually thickened,number of internal cavity increased,and total space increased.The grayish brown or light yellowish dentate pileus were originated from the hyphae tissue at the edge of tissue.After the matte layer was mature,part fence-like cell layers changed to ordinary filamentous hyphae closing to each other and intertwined in the dentate pileus.At last,individual fruiting body similar to that at natural state formed.Ten kinds of liposoluble components were detected by GC-MS in different growth stages of F. trogii and eight were detected at the mycelium pellets stage.Ten were detected at the final context tissue stage and eight were detected at the basidiocarp stage.A total of 56 liposoluble components were detected. 【Conclusion】F. trogii formed basidiocarp similar to natural state through three stages of context tissue differentiation,mediostratum and hymenium differentiation and change in mycelia.The contents and types of lipsoluble components were different in different growth periods. |
| Key words: Funalia trogii biological characteristics GC-MS analysis microscopic structure basidiomata |
