| 摘要: |
| 【目的】优化红缘拟层孔菌多糖的超声提取工艺，为其工业化生产和综合利用提供依据。【方法】以红缘拟层孔菌为材料，考察超声功率、提取温度、液（mL）料(g)比和提取时间对红缘拟层孔菌多糖得率的影响；在此基础上，通过Box-Behnken响应面法，设计4因素3水平试验，建立多糖提取回归方程，确定其最佳提取工艺。【结果】超声功率、提取温度、液(mL)料(g)比和提取时间4个因素对红缘拟层孔菌多糖提取的影响大小依次为：提取温度>超声功率>提取时间>液料比，在单因素试验及响应面优化基础上，确定红缘拟层孔菌多糖的最佳提取工艺为：超声功率60 W，提取温度63 ℃，液料比21∶1，提取时间32 min；在此条件下，多糖得率为12.87%，纯度为69.42%。【结论】得到了红缘拟层孔菌多糖超声提取的优化工艺，该工艺方便可行。 |
| 关键词: 红缘拟层孔菌 多糖 超声提取 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(81660759) |
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| Optimization of ultrasonic extraction of polysaccharides from Fomitopsis pinicola by response surface methodology |
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WANG Yumei
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| Abstract: |
| 【Objective】Ultrasonic extraction of polysaccharides from Fomitopsis pinicola was optimized to provide basis for its development and application.【Method】The effects of ultrasonic power extraction,temperature,liquid (mL)-solid (g) ratio and extraction time on yield of F. pinicola polysaccharide were investigated.Then,experiments were designed based on Box-Behnken response surface with 4 factors and 3 levels,and the regression equation of polysaccharide extraction was established to determine the optimum extraction conditions.【Result】The effects of the 4 factors on the extraction of polysaccharides from red marginal bacteria were in the order of ultrasonic temperature>power extraction>extraction time>liquid(mL)-solid (g) ratio.On the basis of single factor test and response surface optimization,the optimum extraction conditions for polysaccharide extraction from red border were ultrasonic power 60 W,extraction temperature 63 ℃,solid to liquid ratio 21∶1,and extraction time 32 min.The obtained yield of polysaccharides was 12.87% and the purity was 69.42%.【Conclusion】The optimal ultrasonic extraction technology for polysaccharides from F. pinicola was convenient and feasible. |
| Key words: Fomitopsis pinicola polysaccharides ultrasonic extraction |
