| 摘要: |
| 【目的】探究苦参碱对体外培养的奶牛乳腺上皮细胞（BMECs）增殖、凋亡及抗氧化能力的影响。【方法】利用含0（A组），25（B组），50（C组），75（D组）和100 μg/mL（E组）苦参碱的培养基培养奶牛乳腺上皮细胞。通过四甲基偶氮唑盐（MTT）法检测BMECs活性，采用流式细胞仪（AnnexinV/PI双染法）检测苦参碱对BMECs凋亡的影响，并检测苦参碱对BMECs抗氧化酶活性及丙二醛（MDA）含量的影响，采用real-time PCR对BMECs中Caspase-3、p53、STAT1和SOCS3基因的相对表达量进行检测。【结果】用药5 d时，低质量浓度（25和50 μg/mL）苦参碱对BMECs增殖具有促进作用，高质量浓度（75 和100 μg/mL）苦参碱对细胞增殖具有抑制作用；B~E组BMECs的凋亡率均极显著高于A组（P＜0.01）；B~E组BMECs培养上清液中NO和乳酸脱氢酶（LDH）水平明显高于A组。B~E组BMECs的过氧化氢酶（CAT）活性均比A组高，其中C组极显著高于A组（P＜0.01）；B~E组的谷胱甘肽过氧化物酶（GSH-Px）活性均极显著高于A组（P＜0.01），E组的超氧化物歧化酶（SOD）水平极显著高于A组（P＜0.01），各组MDA含量无显著性差异。与A组相比，苦参碱上调了B~E组BMECs中Caspase-3、p53、STAT1和SOCS3基因的相对表达量。【结论】低质量浓度苦参碱能够促进BMECs增殖，高质量浓度苦参碱则会抑制BMECs增殖；不同质量浓度苦参碱均可提高BMECs的抗氧化能力，其中50 μg/mL苦参碱提高BMECs抗氧化能力的效果最明显。 |
| 关键词: 苦参碱 奶牛乳腺上皮细胞 细胞增殖 细胞凋亡 抗氧化能力 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(31460676) |
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| Effect of matrine on proliferation,apoptosis and antioxidant ability of bovine mammary epithelial cells |
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LU Jinxia,HE Fang,FENG Feng,et al
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| Abstract: |
| 【Objective】 This experiment was conducted to study the effect of matrine on proliferation,apoptosis and antioxidant ability of bovine mammary epithelial cells cultured in vitro.【Method】 Groups A,B,C,D and E were set up and treated with matrine at concentrations of 0,25,50,75 and 100 μg/mL,respectively.The activity of BMECs was detected by MTT assay.The effect of matrine on apoptosis of BMECs was detected by flow cytometry (Annexin V/PI double staining).The effect on antioxidase activity and malondialdehyde (MDA) content were also detected.The relative expressions of Caspase-3,p53,STAT1 and SOCS3 in BMECs were detected by real-time PCR.【Result】 Low concentrations of matrine (25 and 50 μg/mL) promoted cell proliferation after 5 days,while high concentrations (75 and 100 μg/mL) inhibited cell proliferation.The apoptosis rates of groups B-E were significantly higher than that of group A (P＜0.01).The levels of NO and LDH of groups B-E were significantly higher than those of group A (P＜0.01).The CAT activities of groups B－E were higher than that of group A and there was significant difference between group C and group A (P＜0.01).GSH-Px activities of groups B-E were significantly higher than that of group A (P＜0.01).The SOD level of group E was significantly higher than that of group A (P＜0.01).There was no significant difference in MDA content.Compared with group A,matrine increased the relative expressions of Caspase-3,p53,STAT1,and SOCS3 in experimental groups. 【Conclusion】 Low matrine concentrations promoted the proliferation of bovine mammary epithelial cells,while high concentrations inhibited cell proliferation and increased the antioxidant abilities of BMECs.Group C with marine concentration of 50 μg/mL was the most effective. |
| Key words: matrine bovine mammary epithelial cells proliferation apoptosis antioxidant ability |
