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青花菜种质资源苗期耐涝性综合鉴定及评价
高 旭1, 张志仙1, 朱长志,等1
台州市农业科学研究院
摘要:
【目的】筛选耐涝的青花菜种质资源,获得能快速鉴定青花菜耐涝性的主要指标。【方法】选用19份不同的青花菜作为供试材料,待幼苗长至三叶一心时进行淹水处理,淹水高于根基1 cm处,以常规水分管理为对照。淹水胁迫10 d后测定幼苗的地上干鲜质量、地下干鲜质量、株高、不定根数和可溶性糖、可溶性蛋白质、丙二醛(MDA)含量及过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性11个生理生化指标,分析各指标间的相关性。以耐涝系数作为评价指标,基于主成分分析及聚类分析,对19份青花菜材料进行耐涝性综合评定及各耐涝相关指标的筛选。【结果】通过11个生理生化指标综合评价可知,炎秀、台绿3号和优秀为耐涝的种质资源。通过主成分分析,可从11个耐涝指标中提取出地下鲜质量、超氧化物歧化酶(SOD)、可溶性糖含量、株高、不定根数以及丙二醛含量6个快速鉴定青花菜苗期耐涝性的指标。各材料综合因子得分(Y值)经聚类分析后,在欧式距离为5时,可将19 份不同青花菜材料按照耐涝性强弱归为3大类。【结论】筛选出了耐涝的青花菜种质资源,确定了鉴定其耐涝性的指标。
关键词:  青花菜  耐涝性  苗期  种质资源  主成分分析  聚类分析
DOI:
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基金项目:浙江省农业(蔬菜)新品种选育重大科技专项(2016C02051-5-2);浙江省重点创新团队项目(2013TD05);浙江省重大科技专项(2014C02006);台州市农业重大专项(14ZD05);台州市重点科技创新团队项目(2014-1)
Comprehensive identification and evaluation of waterlogging tolerance during seedling stage of broccoli germplasms
GAO Xu,ZHANG Zhixian,ZHU Changzhi,et al
Abstract:
【Objective】This study screened waterlogging tolerant germplasms of broccoli and obtained major indexes for rapid identification of waterlogging tolerance.【Method】A total of 19 different broccoli materials were treated by waterlogging stress overtopping 1 cm of the root when the broccoli seedling was in three leaves and one half period.After 10 days,11 physiological and biochemical indexes including shoot dry weight,shoot fresh weight,underground dry weight,underground fresh weight,plant height,adventitious root number,soluble sugar content,soluble protein content and MDA content were measured and the correlations among them were analyzed.The comprehensive evaluation and classification of each index relating with waterlogging tolerance were conducted based on principal component analysis and cluster analysis using waterlogging tolerance coefficient as evaluation index.【Result】Yanxiu,Tailü No.3 and Youxiu were identified as waterlogging-tolerant germplasm resources by comprehensive evaluation based on 11 physiological and biochemical indexes.The indexes were classified into 6 component factors by principal component analysis.The 19 different materials were clustered into 3 groups based on comprehensive factor scores (Y-value) at the Euclidean distance of 5.【Conclusion】This study screened waterlogging-tolerant germplasm resources and determined the indexes to identify the waterlogging tolerance.
Key words:  broccoli  waterlogging tolerance  seedling stage  rermplasw resources  principal component analysis  cluster analysis