| 摘要: |
| 【目的】对四倍体马铃薯F1群体杂种的真伪性进行SSR分子标记鉴定，并对群体与其亲本的遗传多样性进行分析，为加工型马铃薯新品种的选育、种质创新和遗传改良提供理论依据。【方法】将淀粉加工型马铃薯品种天薯10号与炸条加工型品种克新17号进行杂交，获得227份F1群体基因型（KT1~KT-227）。采用轮筛法从70对马铃薯SSR引物中筛选扩增条带清晰、差异性较强、具有双亲特异条带的引物，利用筛选出的引物分析227份马铃薯杂交后代单株的杂种真伪性和遗传多样性，利用NTSYS pc 2.1软件分析群体和亲本的遗传关系。【结果】共筛选出7对特异性引物，分别为S180、STI049、STI019、STI005、STI024、STG0016和STI007。7对引物相互补充鉴定出227份株（系）均为真杂种，真杂种率为100.0%。在引物STI019、STG0016、STI005、STI007的扩增结果中，分别有70，58，20和18份单株出现了双亲都没有的新条带，7对引物扩增结果中均出现不同数量单株亲本位点的缺失。供试材料间的相似系数为0.54～1.00，表明供试材料间遗传差异较大，遗传多样性比较丰富。UPGMA聚类分析结果表明，在相似系数为0.62时，基因型KT-150单独聚为第Ⅰ大类，占F1群体的0.44%；其余228份材料聚为第Ⅱ大类；在相似系数为0.67时，第Ⅱ大类又分为2类：第1类偏向父本克新17号，共74份株（系），占F1群体的32.60%；第2类共152份株（系），占66.96%。在相似性系数为0.69时，第Ⅱ大类第2类又分为2个亚类，第1亚类偏向母本天薯10号，共142份，占62.56%，表明F1群体表现为偏母遗传；第2亚类只有10个单株，占F1群体的4.41%。在相似性系数为0.73时，第Ⅱ大类第2类第1亚类又分为2个亚亚类，第1亚亚类67份株（系），第2亚亚类75份株（系）。【结论】KT-150、KT-95、KT-223、KT-97、KT-214、KT-227、KT-234、KT-119、KT-121、KT-205和KT-207这11份基因型与亲本及其他基因型之间相似性较小，重组变异率较高，有望进一步筛选出综合性状优良的加工型马铃薯株（系）。 |
| 关键词: 加工型马铃薯 实生群体 SSR标记 遗传多样性 |
| DOI： |
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| 基金项目:甘肃省自然科学基金项目（1606RJZA178）；现代农业产业技术体系专项（CARS-10）；甘肃省农业生物技术研究与应用开发项目（GNSW-2014-1）；天水市科技支撑计划项目“主粮化马铃薯新品种选育及加工品质分析研究” |
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| SSR identification and genetic diversity of seedling populations of processing potato |
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LI Fangdi,WANG Peng,DOU Junhuan,et al
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| Abstract: |
| 【Objective】In this study,the authenticity of tetraploid potato F1 population hybrids was identified by SSR and genetic diversity of parents and generations was analyzed to provide basis for breeding new potato varieties,innovating germplasm and genetic improvement of processing potatoes.【Method】Starch-type variety ‘Kexin 17’ and fried-type variety ‘Tianshu 10’were crossed to obtain 227 individuals of F1 population (KT-1-KT-227).A round sieving method was used for selecting polymorphic primers.In total,70 SSRs were used for analyzing the polymorphism in parents and SSR polymorphic primers were selected to identify the hybrids authenticity and genetic diversity.NTSYS-pc 2.1 software was applied for analyzing offspring and the genetic relationship between parents.【Result】A total of 7 pairs of SSR polymorphic primers were selected including S180,STI049,STI019,STI005,STI024,STG0016 and STI007.The heterozygous rate of F1 population was 100.0%,and new specific bands were obtained in 70,58,20 and 18 individuals with primers of STI019,STG0016,STI005 and STI007,respectively.The genetic similarity coefficients of the populations were 0.54-1.00,indicating rich genetic diversity.The UPGMA cluster analysis showed that the F1 populations can be grouped into two categories with similarity coefficient of 0.62 based on 7 SSR primers.The genotype KT-150 accounting for 0.44% was grouped into the category Ⅰ,while other 228 individuals were grouped into the category Ⅱ.With the similarity coefficient of 0.67,the category Ⅱ was grouped into two classes. About 32.60% were grouped into class 1, similar to male parent ‘Kexin 17’.There were 66.96% individuals were grouped into class 2. The category Ⅱ class 2 was grouped into two subclasses when the similarity coefficient was 0.69.Subclass 1 had 62.56% individuals and was similar to female parent ‘Tianshu 10’,showing that F1 populations were characterized by hereditary inheritance.Subclass 2 had only 10 individuals,accounting for 4.41%.With the similarity coefficient of 0.73,category Ⅱ class 2 subclass 1 was grouped into two groups:group 1 had 67 individuals and group 2 had 75 individuals.【Conclusion】The similarity of 11 genotypes (KT-150,KT-95,KT-223,KT-97,KT-214,KT-227,KT-234,KT-119,KT-121,KT-205,and KT-207) with parents and other genotypes was small,and the reorganization mutation rate was high.Processing potato varieties (lines) with excellent comprehensive characteristics can be screened by further studies. |
| Key words: processing potato (Solanum tuberosum L.) seedling population SSR marker genetic diversity |
