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紫菜苔叶片和茎杆花青素含量的遗传分析
刘 进, 梅家琴, 钱 伟
西南大学 农学与生物科技学院
摘要:
【目的】探索紫菜苔叶片和茎杆花青素含量的遗传规律,为紫色白菜的育种或改良提供理论指导。【方法】以紫菜薹(Brassica campestris ssp.chinensis var.purpuraria,AA,2n=20)纯系育种材料与菜心(B.campestris ssp.chinensis var.utilis,AA,2n=20)为亲本,构建F1、F2、BC1P1(B1)和BC1P2(B2)群体,对双亲及上述各群体植株苗期叶片和蕾苔期茎杆的花青素含量进行测定,并通过六世代联合分离分析方法,研究叶片和茎杆中花青素的遗传规律。【结果】紫菜苔(P1)整株表现深紫色,其叶片和茎杆中花青素含量分别为(24.27±4.14) mg/kg和(64.22±7.84) mg/kg;而菜心(P2)整株表现绿色,叶片和茎杆中花青素含量分别为(13.02±2.43) mg/kg和(2.58±0.48) mg/kg;杂种F1代叶片表现为浅紫色,而茎杆表现为微紫色,叶片和茎杆中花青素平均含量分别为(15.73±2.50) mg/kg和(24.20±1.00) mg/kg。各分离世代中,叶片和茎杆花青素含量均呈多峰或偏锋分布,符合具有主基因控制的数量性状分布规律。六世代分析中,叶片和茎杆花青素含量遗传的最适模型分别为2MG-ADI和MX2-ADI-ADI,表明叶片和茎杆花青素含量均受两对加性-显性-上位主基因控制,另外茎杆花青素含量也受多基因的影响。控制叶片和茎杆花青素的主基因均在F2群体中具有较高的遗传率,受环境影响较小。【结论】紫菜薹叶片和茎杆花青素的遗传均受2对主基因影响,主基因在F2中的遗传率较高,因此适宜从F2及自交后代中进行紫色植株的选育。
关键词:  紫菜薹  花青素  六世代群体  遗传
DOI:
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基金项目:国家重点基础研究发展计划(“973”计划)项目(2015CB150201);国家自然科学基金项目(31401861)
Inheritance of anthocyanin contents in leaf and stem of Brassica campestris ssp.chinensis var.purpuraria
LIU Jin, MEI Jiaqin, QIAN Wei
Abstract:
【Objective】The present study investigated the inheritance of anthocyanin contents in leaf and stem of Brassica campestris ssp.chinensis var.purpuraria (AA,2n=20) to provide theoretical guidance for the breeding and improvement of purple B.rapa.【Method】B.campestris ssp.chinensis var.purpuraria is one of the typical purple vegetables in B.campestris L.(genome AA,2n=20).One B.campestris ssp.chinensis var.purpuraria line (P1) and one B.campestris ssp.chinensis var.utilis line (P2) were used to construct F1,F2,BC1P1 (B1) and BC1P2(B2) populations.The anthocyanin contents of seedling leaf and bolting stem were measured in these populations and the two patents.Then,the six-generation joint segregation analysis was conducted to investigated the inheritance for leaf and stem anthocyanin contents.【Result】The whole plant of P1 exhibited deep purple with anthocyanin contents of (24.27±4.14) mg/kg and (64.22±7.84) mg/kg in leaf and stem,respectively.While P2 showed green color,with anthocyanin contents of (13.02±2.43) mg/kg and (2.58±0.48) mg/kg in leaf and stem,respectively.The hybrid F1 showed light purple,with anthocyanin contents of (15.73±2.50) mg/kg and (24.20±1.00) mg/kg in leaf and stem,respectively.In all segregating generations,leaf and stem anthocyanin displayed asymmetry peaks or multimodal distributions,suggesting a quantitative trait nature.The optimal genetic models for leaf and stem anthocyanin were 2MG-ADI and MX2-ADI-ADI,respectively.The leaf anthocyanin content was controlled by two major genes with additive-dominant-epistatic interaction,while the stem anthocyanin content was controlled by both the two major genes in additive-dominant-epistatic interaction and polygenes.The major genes controlling leaf and stem anthocyanin contents exhibited high heritability in F2 generation,suggesting very limited environmental influence on the two traits in this population.【Conclusion】The leaf and stem anthocyanin contents are mainly controlled by two major genes with high inheritance in the F2 generation,thus it is suitable to screen purple plants from the F2 population and its self-offspring.
Key words:  Brassica campestris ssp.chinensis var.purpuraria  anthocyanin  six generation group  genetic