| 摘要: |
| 【目的】从杨树根际土壤筛选固氮菌，并研究其生长特性。【方法】在内蒙古的阿尔山国家森林公园自然保护区，采集杨树根际土壤，采用阿须贝无氮培养基分离纯化杨树根际土壤固氮菌。对分离纯化的固氮菌进行形态和生理生化鉴定；用细菌通用引物27f/1492r 对固氮菌16S rDNA序列进行扩增并测序，应用DNAMAN6.0软件进行序列校正及多序列比对，使用MAGE 4中的邻接法（NJ）构建系统发育树。对分离所获固氮菌的生长特性和解磷能力进行测定。【结果】从阿尔山杨树根际土壤中共分离出XY1、XY2、XY5、XY7、XY8、XY14、XY15、XY9等8株固氮菌，菌落形态、生理生化特性鉴定及系统发育树结果显示，以上8株固氮菌分别属于柄杆菌属（Caulobacter）、叶杆菌属（Phyllobacterium）、红球菌属（Rhodococcus）、假单胞菌属（Pseudomonas）、根瘤菌属（Rhizobium）、类芽孢杆菌属（Paenibacillus）、沙雷氏菌属（Serratia）、芽孢杆菌属（Bacillus）。不同固氮菌生长的最适pH和温度有差异。分离得到的固氮菌中，只有XY2和XY7具有解磷活性。【结论】阿尔山自然保护区杨树根际土壤固氮菌资源丰富，其生物学特性有明显差异。 |
| 关键词: 阿尔山 杨树 根际 固氮菌 16S rDNA |
| DOI： |
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| 基金项目:国家自然科学基金项目（31260173） |
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| Diversity and characterization of nitrogen-fixing bacteria in Populus L.rhizosphere in Arxan National Forest Park |
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NIU Yanfang,YAN Wei,CHEN Lihong,et al
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| Abstract: |
| 【Objective】This paper screened nitrogen fixing bacteria from Populus L.rhizosphere and studied their growth characteristics.【Method】In Arxan National Forest Park of Inner Mongolia,nitrogen-fixing bacteria were isolated from soil of Populus L.rhizosphere and purified by Ashby’s medium.Then morphological and physiology biochemical identification was conducted and the 16S rDNA was amplified with primers 27f/1492r and sequenced.Neighbor-joining tree was also built with DNAMAN6.0 and MEGA 4 and the growth and capacity of phosphate solubilization were measured.【Result】Eight nitrogen-fixing bacterium including XY1,XY2,XY5,XY7,XY8,XY14,XY15 and XY9 were isolated in soil of Populus L.rhizosphere.They belonged to Caulobacter,Phyllobacterium,Rhodococcus,Pseudomonas,Rhizobium,Paenibacillus,Serratia and Bacillus,and had different optimum temperatures and pH values.Only XY2 and XY7 had phosphate solubilization capacity.【Conclusion】Nitrogen-fixing bacteria in Populus L. rhizosphere in Arxan National Forest Park had rich diversity,and there were significant differences in their biological characteristics. |
| Key words: Arxan Populus L rhizosphere nitrogen-fixing bacteria 16S rDNA |
