引用本文:
【打印本页】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 359次   下载 371 本文二维码信息
码上扫一扫!
分享到: 微信 更多
85种植物丙酮提取物的抑菌活性筛选
郭恩辉1, 宋 爽2, 韩立荣,等1,3
1.西北农林科技大学 无公害农药研究服务中心;2.西北农林科技大学无公害农药研究服务中心;3.陕西省生物农药工程技术研究中心
摘要:
【目的】研究85种植物丙酮提取物的抑菌活性,以从中筛选到具有开发潜力的植物源杀菌剂。【方法】以番茄灰霉病菌(Botrytis cinerea)、辣椒疫霉病菌(Phytophthora capsici)、黄瓜炭疽病菌(Colletotrichum orbiculare)为供试病原真菌,采用生长速率法测定85种植物丙酮提取物的抑菌活性,进一步选择皿内测试中抑菌效果较好的植物样品进行活体组织和盆栽药效防治试验。【结果】离体条件及0.1 g/mL剂量下,蓼子朴丙酮提取物对3种供试真菌的抑制率均达到65%以上,披针叶野决明、木姜子、木碱蓬、沙芥、崖棕的丙酮提取物至少对2种病原真菌的抑制率超过65%;活体条件下,蓼子朴、崖棕、木姜子、披针叶野决明丙酮提取物对番茄灰霉病的室内防效均达到50%以上,蓼子朴、脓疮草、白苞蒿、木碱蓬、木姜子、麻黄丙酮提取物对黄瓜炭疽病的室内防效均在50%以上。【结论】蓼子朴具有开发为植物源杀菌剂的潜力,披针叶野决明、崖棕抑菌活性值得进一步研究。
关键词:  植物源杀菌剂;抑菌活性;蓼子朴;番茄灰霉病菌  辣椒疫霉病菌  黄瓜炭疽病菌
DOI:
分类号:
基金项目:国家科技支撑计划项目(2014BAD23B01);杨凌示范区产学研用协同创新重大项目(2016CXY-13)
Antimicrobial activity screening of acetone extracts from 85 plants
GUO Enhui,SONG Shuang,HAN Lirong,et al
Abstract:
【Objective】The antifungal activities of 85 plant acetone extracts were screened to find new botanical fungicides.【Method】The antifungal activities were tested on the concentration of 0.1 g/mL against Botrytis cinerea,Phytophthora capsici and Colletotrichum orbiculare in vitro.Plants with high antifungal effects in vitro were tested about the control effects on C.orbiculare in potted plant materials and on B.cinerea in cucumber cotyledon spray inoculation test.【Result】Extracts from Inula salsoloides presented >65% inhibitory rate to all tested fungi,and the inhibitory rates of extracts from Thermopsis lanceolate,Litsea pungens,Suaeda dendroides,Pugilnium cornutum,Carex siderosticta were >65% against at least two tested phytopathogens.In the tissue test,the extracts from I.salsoloides,C.siderosticta,L.pungens and T.lanceolata had >50% control effect on B.cinerea,and the control effects of extracts from I.salsoloides,Panzeria alaschanica,Artemisia lactiflora,Suaeda dendroides,L.pungens and Ephedra sinica on C.orbiculare were >50%.【Conclusion】I.salsoloidesis able to be explored as new botanical fungicide and it is necessary to further study the antifungal effects of C.siderosticta and T.lanceolate.
Key words:  botanical fungicides  antifungal activity  Inula salsoloides  Botrytis cinerea  Phytophthora capsici  Colletotrichum orbiculare