| 摘要: |
| 【目的】探究花粉 柱头相互作用及主要保护酶、内源激素与牡丹芍药远缘杂交不亲和性的关系，为阐明不亲和的生理机制奠定基础。【方法】对芍药与牡丹杂交授粉及芍药自交授粉过程中，花粉在雌蕊上不同时间（1，3，5，8，12，24，36，48，72，96 h)的生长动态进行荧光显微观察，并对此过程中雌蕊内的保护酶（SOD、POD）活性和内源激素（ZR、ABA、IAA、GA3）含量变化进行比较，分析其形态变化的内在生理机制。【结果】芍药自交授粉后花粉大量萌发，花粉管伸长速度先慢后快，并通过花柱基部；芍药与牡丹杂交后，花粉大部分不能萌发，并在柱头上出现扭曲、肿胀等现象，且产生强烈的胼胝质反应。自交亲和及杂交不亲和授粉雌蕊的POD和SOD活性在授粉过程中发生明显变化，且2种雌蕊的变化趋势存在明显差异；当POD和SOD处于高活性时，花粉管迅速向胚囊伸长并进入胚囊（授粉后3 h）。高含量的ZR、IAA和GA3有利于花粉 柱头识别、萌发和受精作用（授粉后3 h）；整个受精过程中，自交亲和授粉雌蕊的IAA和GA3含量均显著高于杂交不亲和授粉雌蕊，而杂交授粉雌蕊中的ABA含量高于自交授粉，说明高含量的ABA与杂交不亲和性相关。【结论】芍药与牡丹远缘杂交过程中的不亲和性与内部酶活性变化及内源激素含量的动态变化有关。 |
| 关键词: 牡丹 芍药 远缘杂交 保护酶活性 内源激素 |
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| 基金项目:国家自然科学基金项目（31600568，31140057）；河南农业大学科技创新基金项目（30600965）；河南农业大学博士启动基金项目（30600408） |
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| Analysis of pollination affinity performance and its physiological mechanism in Paeonia suffruticosa and Paeonia lactiflora |
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HE Dan,XIE Mengjun,Lü Boya,et al
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| Abstract: |
| 【Objective】This paper explored the pollen-stigma interaction and the relationship between the dynamic changes of protective enzymes and endogenous hormones in pistil during pollinations of Paeonia lactiflora×P.suffruticosa.【Method】The development at different time(1,3,5,8,12,24,36,48,72,96 h) of pollen in pistil was observed under fluoroscope,to compare the dynamic changes of protective enzymes(SOD,POD) active and endogenous hormones(ZR,ABA,IAA,GA3)and analysis of the morphological changes of internal physiological mechanism were studied during pollinations (P.lactiflora×P.suffruticosa and P.lactiflora×P.lactiflora).【Result】The results showed that a large proportion of pollen was germinated and the pollen tube grew slowly at first then fast and entered into the style base after self pollination of P.lactiflora;The pollen was not germinated,and callose largely deposited in papillose cells,and the pollen tube was distortion and swollen on the stigma in P.lactiflora×P.suffruticosa.The co-changes of protective enzymes and endogenous hormones of pistil inside were caused by pollination compatibility,which varied with pollen development;The pollen tube elongated and entered the embryo sac quickly with the high level of POD and SOD (3 h after pollination).The high contents of ZR，IAA and GA3 were beneficial to compatible pollen germination and fertilization (3 h after pollination).The content of ABA of cross-pollination was higher than self-pollination indicating that the high level of ABA was related with cross-incompatibility.【Conclusion】Incompatibility with the dynamic changes of the internal indicators were studied during pollinations (P.lactiflora×P.suffruticosa) laid a solid foundation to elucidate its physiological mechanism. |
| Key words: Paeonia suffruticosa Paeonia lactiflora distant hybridization protective enzymes endogenous hormones |
