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桢楠精油、精气化学成分及精油生物活性研究
丁 文,宁莉萍,杨 威,等
作者单位
丁 文 四川农业大学 林学院 
宁莉萍 四川农业大学 林学院 
杨 威,等 中国林产工业协会 楠木保护与发展促进会 
摘要:
【目的】研究楠木(Phoebe zhennan)(桢楠)精油、精气的化学成分及其生物活性,为楠木养生价值的开发利用提供参考。【方法】以四川桢楠为材料,通过水蒸气法提取桢楠精油,使用气相色谱质谱联用仪分析桢楠精油、精气的化学成分;以白血病HL-60株、肺癌A-549细胞株、肝癌SMMC-7721细胞株、乳腺癌MCF-7细胞株和结肠癌 SW480细胞株等5种癌细胞株和大肠杆菌(ATCC25922)、金黄色葡萄球菌(ATCC29213)、肠沙门氏菌(ATCC14028)、铜绿假单胞菌(ATCC27853)等4种细菌为待测对象,评价桢楠精油的抗肿瘤和抑菌活性。【结果】桢楠精油主要成分为沉香螺旋醇,其相对含量为28.26%;其次为愈创木醇,其相对含量为21.84%;第三是γ 桉叶醇,其含量为8.98%。精气主要成分为沉香螺旋醇,其相对含量为11.93%;其次为α-姜黄烯和愈创木醇,其相对含量分别为7.08%和6.67%。桢楠精油对白血病HL-60细胞株、肺癌A-549细胞株等5种癌细胞有较好的抑制作用,IC50均在50 μg/mL以内;同时,桢楠精油对供试的革兰氏阴性菌和阳性菌均具有抑制作用。【结论】桢楠精油、精气成分有一定相似性,但精气成分更加复杂;桢楠精油对5种癌细胞株有显著抑制作用,但对4种受试细菌的抑菌效果差异较大,且抑菌效果不明显。
关键词:  桢楠  化学成分  生物活性  抑菌作用  抗肿瘤活性
DOI:
分类号:
基金项目:国家“十二五”科技支撑计划项目(2011BAC09B05);四川省“十二五”农作物育种攻关项目(2011NZ0098-10)
Study on chemical compositions of phytoncidere and essential oil and bioactivity of essential oil from Phoebe zhennan
DING Wen,NING Liping,YANG Wei,et al
Abstract:
【Objective】The study on the chemical compositions of the phytoncidere and the essential oil and the bioactivity of essential oil from Phoebe zhennan provides a theoretical basis and reference for exploiting and utilizing the health preservation effects of P.zhennan.【Method】P.zhennan in Sichuan was taken as a research object in this work.The essential oil from P.zhennan was obtained with steam distillation extraction.The chemical compositions of the phytoncidere and the essential oil from P.zhennan were identified by GC-MS analysis,and the bioactivity of essential oil was evaluated by multifunctional microplate reader.【Result】The results show that the main components of the essential oil from P.zhennan were Agarospirol whose content was 28.26%,Guaiol whose content was 21.84%,and γ- Eudesmol whose content was 8.98%.While the main chemical constituents of the phytoncidere from P.zhennan were Agarospirol whose content was 11.93%,α-Curcumene whose content was 7.08%,and Guaiol whose content was 6.67%.The essential oil of P.zhennan on the leukemia HL-60 cell line,lung cancer A-549 cell lines and other three kinds of cancer cells have a good inhibitory effect, and IC50 were within 50 μg/mL;at the same time,the essential oil has inhibitory effect on the gram negative bacteria and positive bacteria of the test.【Conclusion】The chemical composition of the essential oil and the phytoncidere from P.zhennan are a certain similarity,while the compositions of the phytoncidere are more complex.The essential oil from P.zhennan has significant inhibition effects on the five cancer cell lines.However,the antibacterial effects of the essential oil on the four tested bacteria are different,and the antibacterial effect is not obvious. 
Key words:  Phoebe zhennan  chemical compositions  bioactivity  antibacterial action  antitumor activity