| 摘要: |
| 【目的】优化珊瑚菌三萜的微波提取工艺，为珊瑚菌三萜的工业化生产和综合利用提供理论依据。【方法】以珊瑚菌三萜提取率为响应值，考察提取时间、微波温度、乙醇体积分数、料(g)液(mL)比和微波功率对珊瑚菌三萜提取率的影响,在单因素试验基础上，通过 Box-Behnken响应面法,确定其最佳提取工艺。【结果】珊瑚菌三萜的最佳提取工艺条件为微波功率500 W、微波温度50 ℃、乙醇体积分数80%、微波时间150 s、料液比1∶30，在此条件下，三萜提取率为1.320%。【结论】利用Box Behnken响应面设计法得到了珊瑚菌三萜微波提取优化工艺，且该工艺方便可行。 |
| 关键词: 珊瑚菌 三萜 微波辅助提取 单因素试验 Box-Behnken响应面 |
| DOI： |
| 分类号: |
| 基金项目:国家“863”项目“松针、葛根中黄酮等功能性提取物的创制与应用”（2013BAD10B04-3） |
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| Microwave assisted extraction of triterpenoids from Ramaria botrytoides |
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JIN Weili,YANG Licong,XU Haixia,et al
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| Abstract: |
| 【Objective】Microwave-assisted extraction technology of triterpenoids from Ramaria botrytoides was optimized to provide basis for development and application of triterpenoids in Ramaria botrytoides.【Method】With the yield of triterpene as index,effects of microwave power,microwave temperature,ethanol volume fraction,microwave time,and solid to liquid ratio were investigated by single factor tests and Box Behnken response surface methodology.【Result】Optimum microwave extraction technology was:microwave extraction time 150 s,microwave temperature 50 ℃,solid(g) to liquid ratio(mL)1∶30,microwave power 500 W,and ethanol volume fraction 80%. The obtained extraction yield of triterpenoids was 1.320%.【Conclusion】Microwave assisted extraction technology of triterpenoids from Ramaria botrytoides was convenient and feasible. |
| Key words: Ramaria botrytoides triterpenoids microwave extraction single factor test Box-Behnken response surface methodology |
