| 摘要: |
| 【目的】从日本五针松(Pinus parviflora)栽培圃地调查松褐天牛(Monochumus alternatus),并取样镜检松材线虫(Bursaphelenchus xylophilus)，揭示日本五针松感染松材线虫病的可能性和扩散风险, 为开展检疫提供理论依据。【方法】在日本五针松圃地采集枯死或濒死的日本五针松植株，采用分离镜检法检测枯死或濒死日本五针松木质部的线虫种类;剖木检查天牛危害，观察留在日本五针松松枝上的补充营养伤口，同时鉴定天牛幼虫特征，解剖检验羽化的天牛成虫携带LⅣ状态。【结果】在枯死或濒死6株日本五针松枝条样本中有3株样本分离到松材线虫或拟松材线虫，检出率为50%，其中1个样本检出纯松材线虫，1个样本检出纯拟松材线虫，1个样本为松材线虫和拟松材线虫混合寄生，检出率各为33%。侵入枝干的天牛幼虫为松褐天牛幼虫，该幼虫能在日本五针松上顺利羽化为成虫, 并携带松材线虫LⅣ型耐久型幼虫。 【结论】日本五针松能被携带松材线虫的松褐天牛危害并感染松材线虫病,应加强检疫管理, 杜绝通过人为调运苗木扩散传播松材线虫病。 |
| 关键词: 松材线虫 拟松材线虫 松褐天牛 日本五针松 扩散风险 |
| DOI： |
| 分类号: |
| 基金项目:国家“863”计划项目（2012AA101503） |
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| Dispersal risk and identification of pine wood nematode Bursaphelenchus xylophilus (Nematoda:Aphelenchoididae) infected Japanese five needle pine（Pinus parviflora） |
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LAI Yanxue,YANG Zhongqi,WANG Xiaoyi,et al
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| Abstract: |
| 【Objective】This study researched longhorn beetles and nematode from Japanese five-needle pine (Pinus parviflora) garden to reveal the possibility and spread risk of this pine trees infected by pine wood nematode (Bursaphelenchus xylophilus) and provide theoretical support for quarantine.【Method】The wood and twig samples of dead or dying five needle pine trees were collected from nursery.The woods for inspection of longhorn larvae were split and the longhorn beetle feeding scars on the twigs were examined.Some wood samples were carried to lab by isolating and identifying the nematode and then the longhorn larvae in the wood were further identified under dissect microscope.Other wood samples were kept on a box till the larvae developed into adults and then the adults were captured and dissected under microscope to check whether nematodes were carried.【Result】About 33% of the samples were infected by B.xylophilus;and 33% by B.mucronatus.The larvae were identified as M.alternatus,and the adults after emerging were further identified as the beetle M.alternatus,which carried nematodes LⅣ of B.xylophilus.【Conclusion】The Japanese five needle pine trees could be infested by pine wood nematode B.xylophilus and associated with the vector beetle M.alternatus.The pine trees must be quarantined strictly to avoid dispersal of the pine wilt disease. |
| Key words: Bursaphelenchus xylophilus B.mucronatus Monochamus alternatus Pinus parviflora dispersal risk |
