| 摘要: |
| 【目的】探究EGCG（表没食子儿茶素没食子酸酯）与杨梅素的相互作用对绿茶茶汤色泽呈现的影响。【方法】以茶叶中主要的多酚类物质EGCG和杨梅素作为研究对象，将单独的EGCG、杨梅素以及EGCG与杨梅素混合母液分别溶于pH 5.0磷酸缓冲液，在100 ℃水浴中热处理10 h，观察其颜色变化，采用色差仪、紫外分光光度计检测3种溶液的颜色变化规律，并根据溶液的色度值结合动力学方程,建立3种溶液颜色变化的动力学模型。【结果】光谱及色度变化结果显示：EGCG与杨梅素混合母液的色泽变化较其单独母液明显，从淡黄色变成红色；EGCG与杨梅素的相互作用能加深色度的变化，且两者均对混合母液的黄变、红变起作用。互作试验表明，杨梅素能加速EGCG黄变、红变的趋势，EGCG对杨梅素色泽变化有辅助作用。结合颜色动力学分析结果表明，在色泽变化中杨梅素对混合母液的红变起到主要作用，EGCG对混合母液的黄变起到主要作用。【结论】EGCG与杨梅素对色泽变化存在互作效应。 |
| 关键词: EGCG 杨梅素 颜色参数 茶汤色泽 动力学分析 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（31101355） |
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| Effect of EGCG and myricetin interaction on tea color |
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HE Yuanyuan,WANG Jing,YANG Yue,et al
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| Abstract: |
| 【Objective】This study explored the effect of EGCG and myricetin interaction on green tea color.【Method】EGCG and myricetin,the major polyphenols in tea,and their mixture were dissolved in phosphate buffer (pH 5.0) before heat treatment for 10 h in 100 ℃ water bath.Color parameters and color changes of these solutions were monitored by colorimeter and UV.Dynamics models were established based on chroma value and dynamic equation.【Result】The color of mixed solution changed more dramatically from light yellow to red than single solutions.Both EGCG and myricetin had yellow and red effect,and the interaction of them deepened the color change.Myricetin accelerated the variation of yellow and red,and EGCG played a supporting role in color changing of myricetin.Myricetin dominated the red degree of mixed solution,while EGCG contributed to yellow degree.【Conclusion】EGCG and myricetin had synergistic effect on tea color changing. |
| Key words: EGCG myricetin color parameters tea color dynamic analysis |
