| 摘要: |
| 【目的】通过DON暴露体内试验，根据雏鸡脑组织中神经递质含量及钙调蛋白（CAM）含量的变化，研究DON对雏鸡的神经毒性作用。【方法】选取120只健康1日龄海兰蛋鸡（公）预饲1周后，按单因素试验设计随机均分为DON低、中、高剂量组和对照组，对照组和试验组饲喂相同的全价日粮。DON低剂量组、中剂量组和高剂量组分别按采食量中含DON 0.27，1.68和12.21 mg/kg的剂量灌胃，对照组灌服等量的生理盐水。试验从第8天开始，每隔7 d染毒1次，共染毒5次。试验期为36 d。试验结束时，每组随机屠宰20只鸡，迅速取出脑组织，检测神经递质含量和CAM含量的变化。【结果】经高效液相色谱 荧光检测法（HPLC-FD）检测，雏鸡脑组织中未发现5-羟色胺吲哚乙酸（5-HIAA）；与对照组相比，DON高剂量组雏鸡脑组织中去甲肾上腺素（NE）和5-羟色胺（5-HT）含量显著升高（P＜0.05），而多巴胺（DA）含量显著降低（P＜0.05）。与对照组相比，DON中、高剂量组雏鸡神经细胞内游离钙（［Ca2+］i）浓度和脑组织中CAM含量显著降低（P＜0.05），不同DON剂量组CAM mRNA基因相对表达量均显著降低（P＜0.05）。【结论】DON暴露可引起雏鸡神经钙稳态变化，并影响部分神经递质的分泌，对雏鸡具有一定的神经毒性作用。 |
| 关键词: 脱氧雪腐镰刀菌烯醇 雏鸡 脑组织 神经递质 钙调蛋白 |
| DOI： |
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| 基金项目:国家自然科学基金项目（31472250）；安徽省教育厅重点项目（KJ2013A119） |
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| Effects of deoxynivalenol exposure on neurotransmitter and calmodulin contents in chicken |
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GENG Fangfang,FAN Mengxue,JIANG Yunjing,et al
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| Abstract: |
| 【Objective】The study investigated the nerve toxicological effects of deoxynivalenol (DON) on chicken based on changes of neurotransmitter and calmodulin (CAM) contents in brain tissues of chicken through in vivo DON exposure.【Method】A total of 120 Hailan chicken (male,1 day old) were selected and equally divided into low,medium and high DON groups according to single factor experiment design after one week feeding.Chicken in control and treatment groups were fed the same diets.Chicken in low,medium and high dose groups were orally fed with DON solutions of 0.27,1.68 and 12.21 mg/kg,respectively.Chicken in control group were fed with the same doses of sodium chloride solution.The trial last for 36 d.All chicken in treatment groups exposed to DON once every seven days for 5 times.At the end of the trial,20 chicken from each group were slaughtered,and brain tissues were collected and weighed immediately to measure the changes of neurotransmitters and CAM.【Result】HPLC with fluorescence detection showed that 5 HIAA was not found in chicken brain tissues.Compared with the control group,the concentrations of NE and 5-HT in brain tissues of high dose group increased significantly（P＜0.05）,while the concentrations of DA decreased significantly (P＜0.05).Compared with the control group,the contents of ［Ca2+］i in nerve cells and CAM in brain tissues of medium and high dose groups decreased significantly (P＜0.05),and the gene expression of CAM mRNA in treatment groups reduced significantly (P＜0.05).【Conclusion】DON exposure changed nerve calcium homeostasis and the secretions of neurotransmitters in brain tissues,indicating that DON had neurotoxin effects to chicken. |
| Key words: deoxynivalenol（DON） chicken brain tissue neurotransmitter calmodulin |
