| 摘要: |
| 【目的】分析核桃（Juglans regia）凋落叶浸提液和腐解液对萝卜（Raphanus sativus）种子萌发及幼苗生长的影响，探讨核桃凋落叶潜在的化感效应。【方法】采用浸提和腐解（20和40 d）两种方式处理核桃凋落叶，得到3种质量浓度（25 mg/mL（低）、50 mg/mL（中）、100 mg/mL（高））核桃凋落叶浸提液和腐解液，以蒸馏水处理为对照（CK），分析不同质量浓度核桃凋落叶浸提液和腐解液处理下萝卜种子萌发、幼苗生长相关生理指标的变化。【结果】与对照相比，核桃凋落叶浸提液和腐解液均降低了萝卜种子的发芽率、发芽指数和发芽势，并对萝卜幼苗的苗高、根长、鲜质量表现出低促高抑的浓度效应；核桃凋落叶浸提液和20，40 d腐解液均提高了萝卜种子内可溶性蛋白和可溶性糖含量，并明显增加了萝卜幼苗叶片丙二醛含量，对幼苗超氧化物歧化酶（SOD）活性具有促进作用，对过氧化氢酶（CAT）活性具有抑制作用，而对幼苗氧化物酶（POD）活性表现为低促高抑的浓度效应。幼苗可溶性蛋白和可溶性糖含量均随处理液质量浓度的增加而升高。核桃凋落叶腐解液对萝卜的化感效应强于浸提液，且40 d腐解液的化感效应强于20 d腐解液。【结论】在一定质量浓度范围内，核桃凋落叶浸提液和腐解液对萝卜种子萌发及幼苗生长具有较明显的抑制作用，且抑制作用随着处理液质量浓度的升高而增强，同时在一定的时间范围内随凋落叶腐解时间的延长而呈增强趋势。 |
| 关键词: 核桃凋落叶 浸提液 腐解 萝卜 化感作用 |
| DOI： |
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| 基金项目:国家“十二五”科技支撑计划项目（2011BAC09B05） |
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| Effect of water extracts and decomposed liquids of walnut leaf litter on seed germination and seedling growth of radish |
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ZHANG Ruyi,HU Hongling,HU Tingxing,et al
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| Abstract: |
| 【Objective】This study aimed at analyzing the influence of water extracts and leaching solution of decomposed walnut leaf litter (Juglans regia) on seed germination and seedling growth of radish (Raphanus sativus) and exploring the potential allelopathic effect of walnut leaf litter.【Method】Walnut leaf litter was treated by extraction and decomposition (20 d and 40 d).Four concentration levels of water extracts and leaching solution of decomposed walnut leaf litter including L (25 mg/mL),M (50 mg/mL),H (100 mg/mL) and CK (treated by distilled water) were used to test their effects on seed germination and seedling growth of radish.【Result】Both water extract of non-decomposed walnut leaf litter and leaching solution of decomposed leaf litter reduced the germination rate,germination index and germination potential of radish seeds compared with control.The height,the root length,and the fresh weight of radish seedlings were increased at low level and decreased at high level.Water extracts and leaching solution improved the contents of soluble protein and soluble sugar of seeds and the malondialdehyde (MDA) content of seedling leaves.The activity of peroxidase (POD) and superoxide dismutase (SOD) in seedling leaves were also promoted,but the activity of catalase (CAT) was suppressed,the activity of peroxidase (POD) in seedling lesves exhibited a concentration effect of low concentration promotion and high concentration inhibition.The contents of soluble protein and soluble sugar increased with the dose.The allelopathic effect of leaching solution of decomposed leaf litter was higher than that of water extracts of non decomposed leaf litter,and that of leaching solution after 40 d decomposition was higher than that of 20 d.【Conclusion】Water extracts and leaching solution of decomposed leaf litter of walnut had significant inhibition on seed germination and seedling growth of radish.The inhibition increased as the increase of concentrations of solution and the inhibition increased as the increase of decomposition time of leaf litter. |
| Key words: walnut leaf litter water extract leaching solution of decomposing leaf litter radish allelopathic effect |
