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GnIH在陆川仔公猪泌尿生殖系统及内分泌免疫系统中的分布
霍孔林1, 李 昀1, 王晓晔,等1
广西大学 动物科学技术学院
摘要:
【目的】从形态学水平和分子水平探究促性腺激素抑制激素(GnIH)在陆川仔公猪泌尿生殖系统和内分泌免疫系统中的分布情况。【方法】采集30日龄健康陆川仔公猪的睾丸、附睾、附睾管、肾、肾上腺、甲状腺、腭扁桃体、脾、淋巴结、胸腺,采用免疫组织化学和半定量RT PCR的方法检测GnIH在其泌尿生殖系统和内分泌免疫系统的分布情况。【结果】免疫组织化学分析结果显示,在睾丸、附睾、附睾管、肾、肾上腺、甲状腺、腭扁桃体、脾、淋巴结、胸腺均有不同数量的GnIH免疫阳性细胞分布。半定量RT-PCR的检测显示,GnIH mRNA 除了在甲状腺、脾和淋巴结无表达外,在其余组织均有不同程度的表达。【结论】GnIH在泌尿生殖系统和内分泌免疫系统分布广泛,提示GnIH在生殖、免疫和内分泌等生理过程中可能有重要作用。
关键词:  GnIH  陆川仔公猪  免疫组织化学  半定量RT-PCR
DOI:
分类号:
基金项目:“大学生创新创业训练计划”国家级创新训练项目(141059332);广西大学基金项目(XJZ14307);国家自然科学基金青年科学基金项目(31402151);广西理工科学实验中心项目(KTYX2014007)
Distribution of gonadotropin-inhibitory hormone (GnIH) in urinary,reproductive,endocrine and immune systems of Luchuan male piglets
HUO Konglin,LI Yun,WANG Xiaoye,et al
Abstract:
【Objective】This study analyzed the distribution of GnIH in urinary,reproductive,endocrine and immune systems of the Luchuan male piglets from the morphology and molecular levels.【Method】Testis,epididymis,ductus epididymis,kidney,adrenal gland,thyroid gland,palatine tonsil,spleen,lymph gland and thymus tissue of 30 d old healthy male piglets were collected,and immuhischemistry (IHC) and semi-quantitative RT-PCR were used to study the distribution and expression of GnIH in urinary,reproductive,endocrine and immune systems.【Result】IHC results showed that GnIH immune positive cells distributed in testis,epididymis,ductus epididymis,kidney,adrenal gland,thyroid gland,palatine tonsil,spleen,lymph node,and thymus.The results of semi-quantitative RT-PCR suggested that GnIH mRNA expression in all tissues except for thyroid gland,spleen and lymph node.【Conclusion】GnIH widely distributes in urinary,reproductive,endocrine and immune systems,indicating that GnIH may play an important role in many physiological processes,such as reproduction,immune and endocrine.
Key words:  GnIH  Luchuan male piglets  IHC  semi-quantitative RT-PCR