| 摘要: |
| 【目的】探索向育苗基质中加入生防菌及腐植酸钾对甜瓜穴盘育苗生长促进作用的影响。【方法】以甜瓜品种“白沙蜜”为供试材料，采用穴盘育苗技术，将菌剂和腐植酸钾单独或配合施用，共设置不施菌剂及腐植酸钾（对照）、单施放线菌菌剂、单施真菌（灰黄青霉）菌剂、放线菌菌剂与真菌菌剂混施、腐植酸钾单施、放线菌菌剂与腐植酸钾配施、真菌菌剂与腐植酸钾配施、放线菌菌剂+真菌菌剂+腐植酸钾配合施用8个处理，测定不同处理对甜瓜苗茎叶、根系生物量、根系体积与表面积等生物学特性以及叶片POD、PAL、PPO活性、MDA含量和净光合速率等生理生化特性的影响。【结果】用菌剂及腐植酸钾处理后，甜瓜穴盘幼苗生物量均高于对照，其中，放线菌与腐植酸钾、灰黄青霉与腐植酸钾配施处理的苗期生物量较对照分别增加34.1%和19.5%；灰黄青霉与腐植酸钾配施处理甜瓜穴盘幼苗根系总长度、总表面积、平均直径、总体积较对照分别增加46.7%，44.5%，12.5%和62.5%；各处理单叶面积及叶片绿色度值较对照均有所增加，其中灰黄青霉单施、灰黄青霉与腐植酸钾配施处理叶面积较对照分别增加37.3%和85.6%，叶片SPAD值较对照分别增加16.7%和13.6%；灰黄青霉与腐植酸钾配施处理叶片净光合速率较对照增加24.1%；单施生防菌剂、腐植酸钾或两者混施，叶片POD、PAL、PPO活性及丙二醛（MDA）含量均低于对照，其中灰黄青霉与腐植酸钾配施处理的叶片POD、PAL、PPO、MDA较对照分别下降 56.5%，12.0%，25.7%和33.3%。【结论】施用生防菌剂及腐植酸钾能促进甜瓜幼苗生长及根系发育，提高光合速率，降低MDA含量，提高甜瓜穴盘育苗质量，其中生防放线菌与真菌灰黄青霉混合接种时的促生作用更为显著。 |
| 关键词: 育苗基质 生防菌 腐植酸钾 甜瓜 促生作用 |
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| 基金项目:安徽宿州市科技计划项目“功能性基质研究与应用”；国家科技支撑计划项目（2012BAD14B11） |
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| Growth promoting effect of biocontrol bacteria and potassium humate in functional nursery substrate on plug seedling of melon |
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SUN Yueyue,MA Junni,LI Yulong,et al
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| Abstract: |
| 【Objective】This study aimed to explore the growth promoting effect of biocontrol agent and potassium humate in nursery substrate on plug seedling of melon.【Method】Using melon variety “Baishami” as test materials,plug seedling technology was used to study the effect of biological agent and potassium humate on biological,physiological and biochemical characteristics of melon seedling including biomass of root and aerial part,total length,total area,total volume of root,activities of POD,PAL and PPO in leaf,content of MDA,leaf chlorophyll value and photosynthetic characteristics.A total of eight treatments were set:no agent and potassium humate treatment (CK),only actinomyces agent treatment (A),only fungi agent treatment (C),actinomyces agent and fungi agent mixed treatment (AC),only potassium humate treatment (F),actinomyces agent and potassium humate mixed treatment (AF),fungi agent and potassium agent mixed treatment (CF),actinomyces agent,fungi agent and potassium humate mixed treatment (ACF).【Result】The biomass of melon seedlings treated by biocontrol agent and potassium humate were all higher than that in CK,with the highest increases of 34.1% and 19.5% in AF and CF.The root total length,total area,average diameter,and total volume in CF were increased by 46.7%,44.5%,12.5% and 62.5%,respectively.The single leaf area and leaf chlorophyll value were increased in all treatments compared with the control treatment.The leaf areas in C and CF were increased by 37.3% and 85.6% and the leaf chlorophyll values in C and CF were increased by 16.7% and 13.6%,respectively.Leaf net photosynthetic rate was increased by 24.1% in CF treatment.No matter only applied them mixed,The leaf POD,PAL and PPO activities and MDA content were all lower in treatments with biocontrol agent,potassium huamte or both. The leaf POD,PAL and PPO activities and MDA content were decreased by 56.5%,12.0%,25.7% and 33.3% in CF.【Conclusion】Appling biocontrol agent and potassium humate would promote growth and root development of melon seedlings,improve photosynthetic rate,decrease MDA content,and promote quality of melon plug seedlings.The growth-promoting effect was more significant when the biocontrol actinomyces and P.griseofulvum were inoculated together. |
| Key words: nursery substrate biocontrol agent potassium humate melon growth-promoting effect |
